Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /HTH_19(antitoxin)
Location 2820515..2821303 Replicon chromosome
Accession NZ_CP065194
Organism Staphylococcus aureus strain GDC6P096P

Toxin (Protein)


Gene name - Uniprot ID A0A2I7Y5B3
Locus tag I3K80_RS13760 Protein ID WP_000525004.1
Coordinates 2820515..2820976 (-) Length 154 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A0E7YIA0
Locus tag I3K80_RS13765 Protein ID WP_000333630.1
Coordinates 2820989..2821303 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3K80_RS13735 2815566..2817500 + 1935 Protein_2665 YSIRK domain-containing triacylglycerol lipase Lip2/Geh -
I3K80_RS13740 2817594..2818799 - 1206 WP_031795352.1 site-specific integrase -
I3K80_RS13745 2818925..2819539 + 615 WP_000191466.1 hypothetical protein -
I3K80_RS13750 2819536..2819682 - 147 WP_074370993.1 hypothetical protein -
I3K80_RS13755 2819913..2820497 - 585 WP_031918390.1 hypothetical protein -
I3K80_RS13760 2820515..2820976 - 462 WP_000525004.1 hypothetical protein Toxin
I3K80_RS13765 2820989..2821303 - 315 WP_000333630.1 helix-turn-helix domain-containing protein Antitoxin
I3K80_RS13770 2821455..2821691 + 237 WP_001121027.1 helix-turn-helix domain-containing protein -
I3K80_RS13775 2821705..2822481 + 777 WP_001148544.1 Rha family transcriptional regulator -
I3K80_RS13780 2822510..2822653 + 144 WP_000939498.1 hypothetical protein -
I3K80_RS13785 2822643..2822852 - 210 WP_000642492.1 hypothetical protein -
I3K80_RS13790 2822908..2823153 + 246 WP_001025401.1 DUF2829 domain-containing protein -
I3K80_RS13795 2823122..2823487 - 366 WP_016170627.1 hypothetical protein -
I3K80_RS13800 2823542..2823757 + 216 WP_001097552.1 hypothetical protein -
I3K80_RS13805 2823782..2824045 + 264 WP_001124160.1 helix-turn-helix domain-containing protein -
I3K80_RS13810 2824058..2824228 + 171 WP_031838382.1 DUF1270 domain-containing protein -
I3K80_RS13815 2824233..2824547 + 315 WP_031838381.1 hypothetical protein -
I3K80_RS13820 2824612..2824914 + 303 WP_031838380.1 DUF2482 family protein -
I3K80_RS13825 2824919..2825179 + 261 WP_000291481.1 DUF1108 family protein -
I3K80_RS13830 2825192..2825728 + 537 WP_000999048.1 host-nuclease inhibitor Gam family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2817594..2839116 21522


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(4-62)


Sequences

Toxin        


Download         Length: 154 a.a.        Molecular weight: 18074.46 Da        Isoelectric Point: 4.6915

>T182294 WP_000525004.1 NZ_CP065194:c2820976-2820515 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Download         Length: 462 bp

>T182294 NZ_CP086391:c2101257-2101081 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12063.56 Da        Isoelectric Point: 6.4736

>AT182294 WP_000333630.1 NZ_CP065194:c2821303-2820989 [Staphylococcus aureus]
MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYADYIRSKRK

Download         Length: 315 bp

>AT182294 NZ_CP086391:c2101035-2100619 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I7Y5B3

Antitoxin

Source ID Structure
AlphaFold DB A0A0E7YIA0

References