Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 1789971..1790485 Replicon chromosome
Accession NZ_CP065194
Organism Staphylococcus aureus strain GDC6P096P

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag I3K80_RS08880 Protein ID WP_000581792.1
Coordinates 1789971..1790180 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag I3K80_RS08885 Protein ID WP_000138932.1
Coordinates 1790192..1790485 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3K80_RS08860 1785482..1786837 - 1356 WP_000611457.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
I3K80_RS08865 1786852..1787922 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
I3K80_RS08870 1788240..1789442 + 1203 WP_001109939.1 rod shape-determining protein RodA -
I3K80_RS08875 1789706..1789843 - 138 WP_000828354.1 hypothetical protein -
I3K80_RS08880 1789971..1790180 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
I3K80_RS08885 1790192..1790485 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
I3K80_RS08890 1790651..1792135 + 1485 WP_000571550.1 cardiolipin synthase -
I3K80_RS08895 1792163..1792810 + 648 WP_001187614.1 HD domain-containing protein -
I3K80_RS08900 1793090..1793410 + 321 WP_001825748.1 hypothetical protein -
I3K80_RS08905 1793407..1794722 - 1316 Protein_1710 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T182289 WP_000581792.1 NZ_CP065194:c1790180-1789971 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T182289 NZ_CP086391:1447406-1447657 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT182289 WP_000138932.1 NZ_CP065194:c1790485-1790192 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT182289 NZ_CP086391:1447654-1447908 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References