Detailed information of TA system TA182288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1770234..1770763
• Replicon: chromosome
• Accession: NZ_CP065194
• Organism: Staphylococcus aureus strain GDC6P096P

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: I3K80_RS08790
• Protein ID: WP_000621175.1
• Coordinates: 1770234..1770596 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: I3K80_RS08795
• Protein ID: WP_000948331.1
• Coordinates: 1770593..1770763 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3K80_RS08770	1767212..1767982	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
I3K80_RS08775	1767957..1768436	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
I3K80_RS08780	1768438..1768764	-	327	WP_001052491.1	anti-sigma factor antagonist	-
I3K80_RS08785	1768883..1769884	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
I3K80_RS08790	1770234..1770596	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I3K80_RS08795	1770593..1770763	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
I3K80_RS08800	1770848..1771996	-	1149	WP_001281139.1	alanine racemase	-
I3K80_RS08805	1772062..1772421	-	360	WP_000581197.1	holo-ACP synthase	-
I3K80_RS08810	1772425..1772916	-	492	WP_001286980.1	PH domain-containing protein	-
I3K80_RS08815	1772909..1774486	-	1578	WP_001294650.1	PH domain-containing protein	-
I3K80_RS08820	1774479..1774958	-	480	WP_001287081.1	hypothetical protein	-
I3K80_RS08825	1775167..1775727	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T182288 WP_000621175.1 NZ_CP065194:c1770596-1770234 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T182288 NZ_CP086391:961714-962112 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT182288 WP_000948331.1 NZ_CP065194:c1770763-1770593 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT182288 NZ_CP086391:961487-961714 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1