Detailed information of TA system TA182273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1567888..1568546
• Replicon: chromosome
• Accession: NZ_CP065178
• Organism: Pectobacterium quasiaquaticum strain A398-S21-F17

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IG605_RS07365
• Protein ID: WP_193408058.1
• Coordinates: 1568118..1568546 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C6C2Z8
• Locus tag: IG605_RS07360
• Protein ID: WP_012765080.1
• Coordinates: 1567888..1568118 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG605_RS07325 (IG605_007310)	1563936..1564094	+	159	WP_189654467.1	hypothetical protein	-
IG605_RS07330 (IG605_007315)	1564241..1565074	+	834	WP_193399489.1	hypothetical protein	-
IG605_RS07335 (IG605_007320)	1565132..1565482	+	351	WP_193399490.1	hypothetical protein	-
IG605_RS07340 (IG605_007325)	1565605..1566078	+	474	WP_193399491.1	DNA repair protein RadC	-
IG605_RS07345 (IG605_007330)	1566118..1566453	+	336	WP_193399492.1	type IV toxin-antitoxin system YeeU family antitoxin	-
IG605_RS07350 (IG605_007335)	1566523..1566843	+	321	WP_193408056.1	TA system toxin CbtA family protein	-
IG605_RS07355 (IG605_007340)	1566961..1567794	+	834	WP_193408057.1	DUF4942 domain-containing protein	-
IG605_RS07360 (IG605_007345)	1567888..1568118	+	231	WP_012765080.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IG605_RS07365 (IG605_007350)	1568118..1568546	+	429	WP_193408058.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IG605_RS07370 (IG605_007355)	1568839..1570275	-	1437	WP_226373479.1	IS1182 family transposase	-
IG605_RS07375 (IG605_007360)	1570541..1570753	-	213	Protein_1413	IS110 family transposase	-
IG605_RS07380 (IG605_007365)	1570842..1571105	+	264	Protein_1414	integrase core domain-containing protein	-
IG605_RS07385 (IG605_007370)	1571135..1571353	+	219	Protein_1415	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	hcp	1529760..1578581	48821

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15848.06 Da
• Isoelectric Point: 7.3552

>T182273 WP_193408058.1 NZ_CP065178:1568118-1568546 [Pectobacterium quasiaquaticum]
MLKYLLDTNICIYTIKNKPQEVRDAFHRHYGQFAISSITLMELIYGAEKSVNPEKNLAVIEGFSARLEVQNYGFDAAVHT
GQIRAELAKQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVPGLRVENWVGRQADNSQSL

Nucleotide

• Length: 429 bp

>T182273 NZ_CP086372:3987415-3987666 [Xanthomonas oryzae pv. oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8694.75 Da
• Isoelectric Point: 4.3063

>AT182273 WP_012765080.1 NZ_CP065178:1567888-1568118 [Pectobacterium quasiaquaticum]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEEDGVTPDFMITREQPDDQIREDF

Nucleotide

• Length: 231 bp

>AT182273 NZ_CP086372:3987110-3987388 [Xanthomonas oryzae pv. oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7V8Q8E4