Detailed information of TA system TA182264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 134416..135050
• Replicon: chromosome
• Accession: NZ_CP065178
• Organism: Pectobacterium quasiaquaticum strain A398-S21-F17

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: IG605_RS00595
• Protein ID: WP_014917023.1
• Coordinates: 134416..134691 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A433N1R7
• Locus tag: IG605_RS00600
• Protein ID: WP_014917022.1
• Coordinates: 134688..135050 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG605_RS00580 (IG605_000570)	131436..132806	-	1371	WP_193400715.1	envelope stress sensor histidine kinase CpxA	-
IG605_RS00585 (IG605_000575)	132803..133501	-	699	WP_193397772.1	envelope stress response regulator transcription factor CpxR	-
IG605_RS00590 (IG605_000580)	133655..134179	+	525	WP_193397771.1	cell-envelope stress modulator CpxP	-
IG605_RS00595 (IG605_000585)	134416..134691	+	276	WP_014917023.1	type II toxin-antitoxin system HicA family toxin	Toxin
IG605_RS00600 (IG605_000590)	134688..135050	+	363	WP_014917022.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IG605_RS00605 (IG605_000595)	135424..136611	+	1188	WP_193397770.1	sugar transporter	-
IG605_RS00610 (IG605_000600)	136694..137596	+	903	WP_116164081.1	CDF family cation-efflux transporter FieF	-
IG605_RS00615 (IG605_000605)	137804..138766	+	963	WP_193407358.1	6-phosphofructokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10050.70 Da
• Isoelectric Point: 11.1875

>T182264 WP_014917023.1 NZ_CP065178:134416-134691 [Pectobacterium quasiaquaticum]
MQGKIASLRKKQRETLIQIFKTPVLSGVKWSNIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T182264 NZ_CP086370:3895582-3895857 [Massilia sp. HC52]
ATGAAAATCCTATGGACCAGCAAGGCGCAGTCTGACCTGGGCAGGCTGTATGCGTTTCTGGCCCCTCTCAACCCCTCGGC
GGCGGCTCGCGCGATGGCGCAACTCTACGCCGCGCCAGAAAAACTATTCGCGAATCCACGCATAGGCGAACAGCTTACTG
GATTCGGGAATCGAGAAGTGCGGCGCATTCTGGTTGGCAAATACGAACTGCGTTACGAGATCCGGGATTCAGCTATTGCC
ATCATCCGCGTTCGCCATACCCGCGAGCAACTGTAA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13504.31 Da
• Isoelectric Point: 4.4969

>AT182264 WP_014917022.1 NZ_CP065178:134688-135050 [Pectobacterium quasiaquaticum]
MTKALNTPNTMVVSGQPAIISYVPEMGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERQISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT182264 NZ_CP086370:3895313-3895579 [Massilia sp. HC52]
ATGAACACGAGAGACACAACGAGCTGGACGGTACGCATCCCACAGCCGCTCGCCGAGCGGATTTCTGCTTTGGCAAGCAG
TTCCCAAATACCGGTGGACTCTATCGTGGAGCAAGCCCTGGTGCTGTGGGCGGAACGCGAGGACCGGATTTATCAAATGA
CCCTGGAAGGTCTTGCGGATGTTGATGCAGGCAGGGTCGTGGACCATTCAGTGATTAAAGCATGGGTTGAGGGCCTGAAT
ACGGAAAATCCCCTACCTTTGCCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A433N1R7