Detailed information of TA system TA182262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4463185..4463740
• Replicon: chromosome
• Accession: NZ_CP065177
• Organism: Pectobacterium quasiaquaticum strain A477-S1-J17

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: IG609_RS20405
• Protein ID: WP_226373283.1
• Coordinates: 4463489..4463740 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: IG609_RS20400
• Protein ID: WP_193397278.1
• Coordinates: 4463185..4463451 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG609_RS20375 (IG609_020360)	4458704..4459255	+	552	WP_193397267.1	TetR/AcrR family transcriptional regulator	-
IG609_RS20380 (IG609_020365)	4459286..4459696	-	411	WP_193397266.1	helix-turn-helix domain-containing protein	-
IG609_RS20385 (IG609_020370)	4459811..4460662	+	852	WP_193397265.1	SDR family oxidoreductase	-
IG609_RS20390 (IG609_020375)	4460756..4461640	-	885	WP_193397264.1	LysR substrate-binding domain-containing protein	-
IG609_RS20395 (IG609_020380)	4461853..4462989	+	1137	WP_193397263.1	alpha/beta hydrolase	-
IG609_RS20400 (IG609_020385)	4463185..4463451	+	267	WP_193397278.1	helix-turn-helix domain-containing protein	Antitoxin
IG609_RS20405	4463489..4463740	+	252	WP_226373283.1	hypothetical protein	Toxin
IG609_RS20410	4463750..4464106	+	357	WP_226373284.1	HipA domain-containing protein	-
IG609_RS20415 (IG609_020400)	4464158..4464520	+	363	Protein_3979	RhuM family protein	-
IG609_RS20420 (IG609_020405)	4464580..4465944	-	1365	WP_180787248.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
IG609_RS20425 (IG609_020410)	4466074..4467708	-	1635	WP_180787247.1	membrane protein insertase YidC	-
IG609_RS20430 (IG609_020415)	4467711..4467968	-	258	WP_010281237.1	membrane protein insertion efficiency factor YidD	-
IG609_RS20435 (IG609_020420)	4467932..4468291	-	360	WP_010302382.1	ribonuclease P protein component	-
IG609_RS20440 (IG609_020425)	4468310..4468450	-	141	WP_005976668.1	50S ribosomal protein L34	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9381.72 Da
• Isoelectric Point: 4.7440

>T182262 WP_226373283.1 NZ_CP065177:4463489-4463740 [Pectobacterium quasiaquaticum]
VHDIKKIEYKTLSEQQLENILTGYLSDAPLSMIDTEDDFRISIAGAQEKTALLYLDDRWCLPLNATPTTHIIKLPIGKIE
SHS

Nucleotide

• Length: 252 bp

>T182262 NZ_CP086370:c1241488-1241075 [Massilia sp. HC52]
ATGATCATTCTCGACACGAACGTTATTTCGGAGGCGATGAAGGCCGCGCCCCACCCTGCGGTCCAAGCCTGGCTGAACAA
TCAGGCCGCCGAGACTCTGTATCTATCCAGCGTTACCCTTGCCGAGCTACTGTTCGGCATCCACTCACTCCCAGCCGGCA
AGCGGCAGGACTTACTGACGCGAGCCCTTGACGCTCTTCTGGGACTATTCAAGGGTCGCATACTTCCTTTTGATACTGAC
GCGGCCCGGCACTACGCCGAACTGGCAGTTGCAGCCAAGGCCGCAGGGCATGGATTTCCCATGCCAGACAGCTACATCGC
CGCCATTGCAGATTCTCGGAAATTCATCGTGGCATCGCGCGACACGGCCCCCTATGAGGCGGCGGGCCTGACGGTTGTCA
ATCCTTGGGCCTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9943.26 Da
• Isoelectric Point: 9.7281

>AT182262 WP_193397278.1 NZ_CP065177:4463185-4463451 [Pectobacterium quasiaquaticum]
MKITNSKQLSSYLKDVRITQKLSQGKVASKVGIRQDTVSSFEQHPDSTKLETFFKILSALNLELTVTPRNVDTINNEASV
ASSWKEEW

Nucleotide

• Length: 267 bp

>AT182262 NZ_CP086370:c1241739-1241485 [Massilia sp. HC52]
ATGGCTATGCTTACAATACGCAACCTACCCGAAGATGTGCACCGTGCATTGCGGGTGAGGGCCGCACTGCACGGGCGCAG
CACCGAGGCGGAGGTTCGTGAAATACTGGCCAGTGCGGTGAAGCCCGAAGCCCGCGTCCGCATGGGAGACGCGCTCGCCG
CCCTGGGACGGGAGATAGGCATCACGGATGACGATCTTGAAACCATGATCCAAACCCGGGACAAAACACCGGCCGAGCCG
CCGAGGTTTGAATGA