Detailed information of TA system TA182258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3580038..3580564
• Replicon: chromosome
• Accession: NZ_CP065177
• Organism: Pectobacterium quasiaquaticum strain A477-S1-J17

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: IG609_RS16375
• Protein ID: WP_193398338.1
• Coordinates: 3580277..3580564 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7V8L5M4
• Locus tag: IG609_RS16370
• Protein ID: WP_039351585.1
• Coordinates: 3580038..3580277 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG609_RS16345	3575069..3575251	+	183	Protein_3181	host cell division inhibitor Icd-like protein	-
IG609_RS16350 (IG609_016340)	3575287..3575484	+	198	WP_182101077.1	host cell division inhibitor Icd-like protein	-
IG609_RS16355 (IG609_016345)	3575477..3575644	+	168	WP_193398340.1	hypothetical protein	-
IG609_RS16360 (IG609_016350)	3575641..3578199	+	2559	WP_193398339.1	TOPRIM and DUF927 domain-containing protein	-
IG609_RS20495	3578805..3578933	+	129	WP_264177029.1	hypothetical protein	-
IG609_RS16365	3579175..3579684	+	510	WP_226374086.1	hypothetical protein	-
IG609_RS16370 (IG609_016360)	3580038..3580277	+	240	WP_039351585.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
IG609_RS16375 (IG609_016365)	3580277..3580564	+	288	WP_193398338.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IG609_RS16380 (IG609_016370)	3580579..3580941	+	363	WP_226374087.1	hypothetical protein	-
IG609_RS16385 (IG609_016375)	3581099..3581532	+	434	Protein_3190	hypothetical protein	-
IG609_RS16390 (IG609_016380)	3581893..3582276	-	384	WP_193398337.1	helix-turn-helix domain-containing protein	-
IG609_RS16395 (IG609_016385)	3582281..3582646	-	366	WP_193398336.1	DUF6516 family protein	-
IG609_RS16400 (IG609_016390)	3582877..3583401	+	525	WP_193398335.1	hypothetical protein	-
IG609_RS16405 (IG609_016395)	3583398..3583598	+	201	WP_193398334.1	AlpA family phage regulatory protein	-
IG609_RS16410 (IG609_016400)	3583564..3583903	-	340	Protein_3195	IS1 family transposase	-
IG609_RS16415 (IG609_016405)	3584044..3584499	-	456	WP_014916544.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3583688..3583903	215

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-78)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11314.19 Da
• Isoelectric Point: 10.1960

>T182258 WP_193398338.1 NZ_CP065177:3580277-3580564 [Pectobacterium quasiaquaticum]
MAYFLDFDERALKEWRKLGETVRNQFKKKLTDILEAPRIEANKLRGMPDCYKIKLRTAGYRLVYQVQDEKVVVFVVSVGK
RERSEAYDDAIKRLR

Nucleotide

• Length: 288 bp

>T182258 NZ_CP086369:c3925154-3924951 [Pectobacterium versatile]
ATGAAAAAAATCGACTATTTGATGCGTTTGCGTAAATGCACAACCATTGACACTCTCGAACGCGTTATTGAAAAAAACAA
GTATGAACTCTCTAATGATGAACTGGAGATGTTCTTTTCCGCAGCCGATCATCGTCTGGCTGAACTGACAATGAACAAAC
TCTACGACAAAGTTCCTACCGCAGTATGGCGATACGTCCGTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9051.37 Da
• Isoelectric Point: 4.5112

>AT182258 WP_039351585.1 NZ_CP065177:3580038-3580277 [Pectobacterium quasiaquaticum]
MGSINLRIDDDLKTRSYAALEKLGVTPSEALRLTLEYIAENERLPFKQALLSDEDAELIEIVRERLRNPKPVRVTLDDL

Nucleotide

• Length: 240 bp

>AT182258 NZ_CP086369:c3925576-3925208 [Pectobacterium versatile]
ATGGATGAGTACACACCAAAACATTATGATATTGCCCAACTCAGATTCTTATGTGAGAATCTGTGTGACGAAAGCATAGC
AACGTTAGGTGATAGCAGTCACGGTTGGGTCAATGATCCAACATCTGCGATCAATCTCCAATTAAATGAACTTATTGAGC
ATATCGCTACGTTTATTCTCACATTTAAAATAAAATACCCTAACGAAAGTGAGCTTTCAGAGCAGGTTGAAAAGTATTTG
GATGATACTTACGTCTTGTTTAGCAACTATGGAATTAATGATGCTGAACTGCGGCGTTGGCAGAAGTCCAAAGCAAAATT
ATTCGGAATGTTCTCAGGGGAGAACGTCTGTACGCCTGCTAAAACTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7V8L5M4