Detailed information of TA system TA182257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3449474..3450252
• Replicon: chromosome
• Accession: NZ_CP065177
• Organism: Pectobacterium quasiaquaticum strain A477-S1-J17

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: IG609_RS15775
• Protein ID: WP_193401168.1
• Coordinates: 3449474..3449962 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A3S0Y1Y3
• Locus tag: IG609_RS15780
• Protein ID: WP_038921611.1
• Coordinates: 3449959..3450252 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG609_RS15755 (IG609_015740)	3445418..3446161	+	744	WP_193401172.1	MobC family replication-relaxation protein	-
IG609_RS15760 (IG609_015745)	3446220..3446438	+	219	WP_012773205.1	type II toxin-antitoxin system antitoxin CcdA	-
IG609_RS15765 (IG609_015750)	3446441..3446746	+	306	WP_193398415.1	type II toxin-antitoxin system toxin CcdB	-
IG609_RS15770 (IG609_015755)	3447127..3449316	-	2190	WP_193401170.1	AAA family ATPase	-
IG609_RS15775 (IG609_015760)	3449474..3449962	-	489	WP_193401168.1	GNAT family N-acetyltransferase	Toxin
IG609_RS15780 (IG609_015765)	3449959..3450252	-	294	WP_038921611.1	DUF1778 domain-containing protein	Antitoxin
IG609_RS15785 (IG609_015770)	3450671..3451930	+	1260	WP_250127221.1	tyrosine-type recombinase/integrase	-
IG609_RS15790 (IG609_015775)	3452823..3453044	-	222	WP_012822470.1	helix-turn-helix transcriptional regulator	-
IG609_RS15795 (IG609_015780)	3453041..3453502	-	462	WP_250127222.1	hypothetical protein	-
IG609_RS15800 (IG609_015785)	3454101..3454700	+	600	WP_193400061.1	TIR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3432736..3466752	34016

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17423.17 Da
• Isoelectric Point: 7.2921

>T182257 WP_193401168.1 NZ_CP065177:c3449962-3449474 [Pectobacterium quasiaquaticum]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVSGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEARDFYLRVGFEPSPIDPMILMVTLGDLVG
SV

Nucleotide

• Length: 489 bp

>T182257 NZ_CP086369:c3668145-3667840 [Pectobacterium versatile]
ATGCAATTCATCGTTTATGGATACAAAAGAGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCCAAGCGGCGCATGGTCATCCCGATTATCGAATCGCATCACCTGTCTGAGAAAGTGAATAAAACATTGTTCCCGCTGA
TCCGCATCGACGGAGAAGGTTATCGACTGATGACCATGGAACTATCGAGCGTTCCGGTTGAGGTTATTGGTGAAGTGATT
GCGGATCTTGGCGACTATTCGGATGAAATTAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10856.44 Da
• Isoelectric Point: 10.1968

>AT182257 WP_038921611.1 NZ_CP065177:c3450252-3449959 [Pectobacterium quasiaquaticum]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT182257 NZ_CP086369:c3668366-3668148 [Pectobacterium versatile]
ATGAAACACCGCGTTAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAATGACGCCATTGGCAAAGAAGCCCGACGCATCAGGGCTGAAGAGTGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCACAGCATGGCTCCTTTGCAGATGAAAACAGGAACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3S0Y1Y3