Detailed information of TA system TA182256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3446220..3446746
• Replicon: chromosome
• Accession: NZ_CP065177
• Organism: Pectobacterium quasiaquaticum strain A477-S1-J17

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: IG609_RS15765
• Protein ID: WP_193398415.1
• Coordinates: 3446441..3446746 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: E0SK21
• Locus tag: IG609_RS15760
• Protein ID: WP_012773205.1
• Coordinates: 3446220..3446438 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG609_RS15740 (IG609_015725)	3442462..3443721	+	1260	WP_193401176.1	integrase arm-type DNA-binding domain-containing protein	-
IG609_RS15745 (IG609_015730)	3443864..3444034	-	171	WP_164669135.1	hypothetical protein	-
IG609_RS15750 (IG609_015735)	3444175..3444516	+	342	WP_193401174.1	hypothetical protein	-
IG609_RS15755 (IG609_015740)	3445418..3446161	+	744	WP_193401172.1	MobC family replication-relaxation protein	-
IG609_RS15760 (IG609_015745)	3446220..3446438	+	219	WP_012773205.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
IG609_RS15765 (IG609_015750)	3446441..3446746	+	306	WP_193398415.1	type II toxin-antitoxin system toxin CcdB	Toxin
IG609_RS15770 (IG609_015755)	3447127..3449316	-	2190	WP_193401170.1	AAA family ATPase	-
IG609_RS15775 (IG609_015760)	3449474..3449962	-	489	WP_193401168.1	GNAT family N-acetyltransferase	-
IG609_RS15780 (IG609_015765)	3449959..3450252	-	294	WP_038921611.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3432736..3466752	34016

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11806.65 Da
• Isoelectric Point: 4.8500

>T182256 WP_193398415.1 NZ_CP065177:3446441-3446746 [Pectobacterium quasiaquaticum]
MQFIVYQYKRTSHYKMFVDVQSDLVETPKRRMVIPLIESHHLSEKVNKTLFPQLRIDGEDYRLMTTELSSVPVEVIGEVI
ADLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T182256 NZ_CP086369:3664062-3664556 [Pectobacterium versatile]
ATGATTTCCGCCCCTGAGCCACTCCACGCCGAACATGTTCTATCGTCATTCTGTTGCGGCGTGGAGTCTATGGACAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTCACTGGTGCATCGCGAACTTTCGTCAGTTGCGATGGCTCCAAAGTGCTGG
CGTATTACTCATTGGCTTCCAGCGCCGTGGCAACGAATGCCGCTCCCGGACGTTTTCGCCGCAATATGCCCGATCCGATC
CCAGTCGTGGTGCTGGGTCGTCTGGCAGTAGATAGATCATTGCATGGTCAGGGTGTTGGCCGTGCTCTGGTACGTGATGC
TGGGCTAAGAGTGATTCAGGTCGCTGATACTATCGGTATTCGCGGGATGTTGGTTCATGCGCTGTCTGATGAGGCGCGGG
AGTTTTACCTACGGGTGGGGTTTGAACCTTCACCAATAGACCCAATGATGCTGATGGTGACGTTGGGGGATTTGGTGGGA
AGTTTATCAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8201.16 Da
• Isoelectric Point: 7.2038

>AT182256 WP_012773205.1 NZ_CP065177:3446220-3446438 [Pectobacterium quasiaquaticum]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQHGSFADENRNW

Nucleotide

• Length: 219 bp

>AT182256 NZ_CP086369:3663772-3664065 [Pectobacterium versatile]
ATGTCAGCGACACACAGCGTTTCAGTTAAACGCGAAACATTGAATTTACGCATTAAGCCCGCAGAACGCGATCTTATCGA
TCGTGCTGCGAAGGCCAGAGGAAAAAATCGCACGGATTTTGTCCTTGAGGCTGCGCGTGCCGCCGCAGAAGAGGCGTTGA
TCGATCAACGCATCATCATGGCAGATCCTGAAGCTTATCAAGAATTCCTTGCGCGCTTGGATCAAGCTCCTTCGCCGAAT
GCCGCGCTGCGCGAAACCATGCAAACCCCTGCACCGTGGGAGCAGAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: E0SK21