Detailed information of TA system TA182255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3404043..3405079
• Replicon: chromosome
• Accession: NZ_CP065177
• Organism: Pectobacterium quasiaquaticum strain A477-S1-J17

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: IG609_RS15575
• Protein ID: WP_235204923.1
• Coordinates: 3404043..3404546 (-)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: IG609_RS15580
• Protein ID: WP_193397972.1
• Coordinates: 3404618..3405079 (-)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG609_RS15555 (IG609_015540)	3401888..3402301	-	414	WP_193398011.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
IG609_RS15560 (IG609_015545)	3402288..3402743	-	456	WP_226374262.1	YgdB family protein	-
IG609_RS15565 (IG609_015550)	3402740..3403291	-	552	WP_250116875.1	prepilin peptidase-dependent protein	-
IG609_RS15570 (IG609_015555)	3403360..3403863	-	504	WP_193397973.1	prepilin peptidase-dependent protein	-
IG609_RS15575 (IG609_015560)	3404043..3404546	-	504	WP_235204923.1	PIN domain-containing protein	Toxin
IG609_RS15580 (IG609_015565)	3404618..3405079	-	462	WP_193397972.1	helix-turn-helix domain-containing protein	Antitoxin
IG609_RS15585 (IG609_015570)	3405301..3406095	-	795	WP_193397971.1	thymidylate synthase	-
IG609_RS15590 (IG609_015575)	3406132..3406998	-	867	WP_193398010.1	prolipoprotein diacylglyceryl transferase	-
IG609_RS15595 (IG609_015580)	3407225..3409471	-	2247	WP_193397970.1	phosphoenolpyruvate--protein phosphotransferase	-
IG609_RS15600 (IG609_015585)	3409484..3410017	-	534	WP_102118309.1	RNA pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-91)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18752.65 Da
• Isoelectric Point: 4.6914

>T182255 WP_235204923.1 NZ_CP065177:c3404546-3404043 [Pectobacterium quasiaquaticum]
MHLGLTGLYQPKWTALIQEEWQRNLLLNNADVTMDKLRNIEHLMNKALPDANVTGFEQLIPALVLPDADDRHVLAAAIRA
NAEVIVTANLKDFPATALSEFAVEAMHPDEFIADLFDLNSALVLKAVRTSRTAFRKPPFDVDEYLDCLLRQGLPMTVKEL
DAYRALI

Nucleotide

• Length: 504 bp

>T182255 NZ_CP086369:3662707-3663201 [Pectobacterium versatile]
ATGATCTCAGCCCCTGAGCCACTTCACGCCGAACATGTATTTTCCTCATTCGGTTGCGGCGTGGAGTCTATGGACAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTCACTGGTGCATCGCGAACTTTTGTCAGTTGCGATGGCTCAAAAGTGCTGG
CCTATTACTCATTGGCCTCCAGTGCTGTAGCAACGAATGCCGCCCCCGGACGTTTTCGCCGCAATATGCCCGATCCGATT
CCGGTGGTAGTGCTGGGACGTCTGGCGGTGGATACGTCATTGCATGGTCAAGGTGTTGGCCGAGCGCTGGTGCGTGATGC
CGGGCTTCGGGTTATTCAGGTAGCGGACACTATCGGCATTCGCGGGATGTTGGTTCATGCGTTGTCTGATGAGGCGCGAA
ATTTTTACCTACGGGTGGGGTTTGAGCCTTCACCGATTGATCCCATGATGTTGATGGTAACGTTGGGGGATTTGGTGGGG
AGTTTATCAATTTGA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17216.74 Da
• Isoelectric Point: 6.2253

>AT182255 WP_193397972.1 NZ_CP065177:c3405079-3404618 [Pectobacterium quasiaquaticum]
MKNTPLNSLSLPAPKEMEAAIRGQRQLATYLSTKLETQEISIQDAHDIAHRIELPTSALTLLMSILGELAAGNAVQIVPV
QAELTTQEAANILNVSRPHMVKLLEEGHLPFHKTGRHRRVLFADLMQYKEQRESESMQAMQELTDRSQELGFY

Nucleotide

• Length: 462 bp

>AT182255 NZ_CP086369:3662417-3662710 [Pectobacterium versatile]
ATGCCAGTGACGAACAGCGTTTCTGTTAAACGCGAAACATTAAATTTACGCATCAAGCCCGCCGAACGAGATCTTATTGA
TCGTGCTGCGAAGGCCAGAGGAAAAAATCGCACTGATTTTGTCCTCGAAGCTGCTCGTGCCGCCGCAGAGGAAGTGTTGA
TCGATCAGCGCATCATCATGGCAGATCCCGATGCTTATCAGGAGTTCCTTGCTCGCTTGGATCAAACTCCCTCGCCGAAT
GCCGCGCTGCGCAAAACCATGCAAACACCTGCACCGTGGGAACAGAAAAAATGA