Detailed information of TA system TA182254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3225168..3225792
• Replicon: chromosome
• Accession: NZ_CP065177
• Organism: Pectobacterium quasiaquaticum strain A477-S1-J17

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: C6DB72
• Locus tag: IG609_RS14765
• Protein ID: WP_005976087.1
• Coordinates: 3225589..3225792 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: IG609_RS14760
• Protein ID: WP_193397543.1
• Coordinates: 3225168..3225536 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG609_RS14745 (IG609_014745)	3220799..3223927	+	3129	WP_193397542.1	efflux RND transporter permease subunit	-
IG609_RS14750 (IG609_014750)	3224160..3224408	+	249	WP_102116757.1	type B 50S ribosomal protein L31	-
IG609_RS14755 (IG609_014755)	3224426..3224569	+	144	WP_072012464.1	type B 50S ribosomal protein L36	-
IG609_RS14760 (IG609_014760)	3225168..3225536	+	369	WP_193397543.1	Hha toxicity modulator TomB	Antitoxin
IG609_RS14765 (IG609_014765)	3225589..3225792	+	204	WP_005976087.1	HHA domain-containing protein	Toxin
IG609_RS14775 (IG609_014775)	3226536..3226856	+	321	WP_010282239.1	MGMT family protein	-
IG609_RS14780 (IG609_014780)	3226876..3227457	-	582	WP_193397544.1	YbaY family lipoprotein	-
IG609_RS14785 (IG609_014785)	3227668..3228531	+	864	WP_193397545.1	acyl-CoA thioesterase II	-
IG609_RS14790 (IG609_014790)	3228683..3229969	-	1287	WP_167469165.1	ammonium transporter AmtB	-
IG609_RS14795 (IG609_014795)	3230009..3230347	-	339	WP_002208627.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8145.52 Da
• Isoelectric Point: 8.9008

>T182254 WP_005976087.1 NZ_CP065177:3225589-3225792 [Pectobacterium quasiaquaticum]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Nucleotide

• Length: 204 bp

>T182254 NZ_CP086369:c3498421-3498170 [Pectobacterium versatile]
ATGAGCTATAAGCTGGAGTTTGAAGAGCACGCGCTGAAAGAATTTAAGAAGCTGAGAGCTCCGATGTGGGAGCAGTTCAC
CAAAAAATTGAAAGAGATTTTACAAAATCCCCACGTTCCCGCCAATCGTCTTCACGGAATGGCTGACTGCTACAAGAAGT
TTATAAAGCAGCCAAAGACCGACTATAAGCGCGGACAGTTTTCAACTCGCGGCTATTTTGTTGTGCAACCTATCTATCAA
GGTGACGAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14102.92 Da
• Isoelectric Point: 4.3697

>AT182254 WP_193397543.1 NZ_CP065177:3225168-3225536 [Pectobacterium quasiaquaticum]
MDEYTPKYYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINLQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELRRWQKSKAKLFGMFSGENICMPAKT

Nucleotide

• Length: 369 bp

>AT182254 NZ_CP086369:c3498662-3498411 [Pectobacterium versatile]
ATGTCCGTACAACCTATCCTTGCAAATGCAGCTGTGAGTATCAGCGACTTTAAAAAATCGCCCAATGCCGCGCTTAAAGA
AGCTAACGGTGAGCCTGTAGCGATGCTCACCAATGGCCGTATCTCTGGCTACTATGTTTCCCCGGAAACATGGGAGGCGC
TGGCCGACTATCAGGAAGATATCGAACTTGCCAAAATCGCCCGCTCACGGATGAAGGGGAAACGTGTGAAGGTCGATCTG
GATGAGCTATAA

Structures

Toxin

• AlphaFold DB: A0A1D7Z3F0