Detailed information of TA system TA182250

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1581315..1582040
• Replicon: chromosome
• Accession: NZ_CP065177
• Organism: Pectobacterium quasiaquaticum strain A477-S1-J17

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: IG609_RS07310
• Protein ID: WP_193399493.1
• Coordinates: 1581720..1582040 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: IG609_RS07305
• Protein ID: WP_193399492.1
• Coordinates: 1581315..1581650 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG609_RS07275 (IG609_007280)	1576911..1577966	+	1056	WP_193399487.1	hypothetical protein	-
IG609_RS07280 (IG609_007285)	1578062..1578949	+	888	WP_193399488.1	50S ribosome-binding GTPase	-
IG609_RS07285 (IG609_007290)	1579133..1579291	+	159	WP_189654467.1	hypothetical protein	-
IG609_RS07290 (IG609_007295)	1579438..1580271	+	834	WP_193399489.1	hypothetical protein	-
IG609_RS07295 (IG609_007300)	1580329..1580679	+	351	WP_193399490.1	hypothetical protein	-
IG609_RS07300 (IG609_007305)	1580802..1581275	+	474	WP_193399491.1	DNA repair protein RadC	-
IG609_RS07305 (IG609_007310)	1581315..1581650	+	336	WP_193399492.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IG609_RS07310 (IG609_007315)	1581720..1582040	+	321	WP_193399493.1	TA system toxin CbtA family protein	Toxin
IG609_RS07315 (IG609_007320)	1582158..1582991	+	834	WP_193399494.1	DUF4942 domain-containing protein	-
IG609_RS07320 (IG609_007325)	1583085..1583315	+	231	WP_104209861.1	type II toxin-antitoxin system VapB family antitoxin	-
IG609_RS07325 (IG609_007330)	1583315..1583719	+	405	WP_193399495.1	tRNA(fMet)-specific endonuclease VapC	-
IG609_RS07330 (IG609_007335)	1584121..1584333	-	213	Protein_1405	IS110 family transposase	-
IG609_RS07335 (IG609_007340)	1584422..1584685	+	264	Protein_1406	integrase core domain-containing protein	-
IG609_RS07340 (IG609_007345)	1584715..1584933	+	219	Protein_1407	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11966.67 Da
• Isoelectric Point: 4.7627

>T182250 WP_193399493.1 NZ_CP065177:1581720-1582040 [Pectobacterium quasiaquaticum]
MQTSPAIPPREDNPCASPVAVWQQLLTYLLEKHYGLTLNDTPFCEENVIQAHIDAGVTLVNAVNFLVEKYELVRIDRDGF
NWQEQSPFLTAVDILRARRATGLLKA

Nucleotide

• Length: 321 bp

>T182250 NZ_CP086369:3155257-3155562 [Pectobacterium versatile]
ATGCCACAAGTGATCGTTTCAGAACGCGCGAGGCGTGATTTGCAGCGTCTACAGGATTTTCTAAAAAACAAAAATGCGCT
GGCCGCGAAAAAGGCCGCTGAAGTTTTGATTCGGGGCATTCGTCAGTTAGAAGCGCTGCCGAATATCGGACGACCAGTGG
AACATCTTCCCCTTGAATATCAGGAGTTGGTGATCGAATTCGGCAGCAGCGGGTACGTGATGCTTTACCGTCACGATGAG
CTGATCGGCAGAGTAGTGATACTGACCGTTAAACATCAAAAAGAAGCCGGTTATCAAAACCCGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12433.12 Da
• Isoelectric Point: 5.1308

>AT182250 WP_193399492.1 NZ_CP065177:1581315-1581650 [Pectobacterium quasiaquaticum]
MPSSDTPEWGLKCAITRRFGARLVQEGNRLHYLADRASIVGTFSKTEAQSLERCFPELIELLEQKLRMGELNPRQQGGVT
LYCNEWTCEADTLGSVGYVYIVIYPSSAATQ

Nucleotide

• Length: 336 bp

>AT182250 NZ_CP086369:3154979-3155269 [Pectobacterium versatile]
ATGGCTGACACCACACGTCAAACCACGACGCTGCGCATTGATCAGGACATTAAAGAACGACTGAAAACGCTGGCCGATGA
CCGCAACAGCTCGGCCCATGCGCTGATGCTGGAAGCGATTGCCGAATACGTTGAGCGGGAAGAAAAACGCAGCCAGTACC
GTAAAGAGGCGTTGGCCGCGTGGGAAGACTATCAGGACACTGGCAAACATATCACCGCAGATGAGACCGTCACTTGGTTA
GAATCCTGGGGTTCTGGACATGAACAGGATGCACCTGCATGCCACAAGTGA