Detailed information of TA system TA182248

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 709644..710304
• Replicon: chromosome
• Accession: NZ_CP065177
• Organism: Pectobacterium quasiaquaticum strain A477-S1-J17

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: IG609_RS03315
• Protein ID: WP_226373417.1
• Coordinates: 709930..710304 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A1D7Z1Z6
• Locus tag: IG609_RS03310
• Protein ID: WP_005971623.1
• Coordinates: 709644..709910 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG609_RS03290 (IG609_003285)	705479..706483	-	1005	WP_193397389.1	LacI family DNA-binding transcriptional regulator	-
IG609_RS03295 (IG609_003290)	706538..707146	-	609	WP_193397388.1	HD domain-containing protein	-
IG609_RS03300 (IG609_003295)	707570..708217	+	648	WP_010279833.1	hemolysin III family protein	-
IG609_RS03305 (IG609_003300)	708408..709409	-	1002	WP_193397387.1	tRNA-modifying protein YgfZ	-
IG609_RS03310 (IG609_003305)	709644..709910	+	267	WP_005971623.1	FAD assembly factor SdhE	Antitoxin
IG609_RS03315 (IG609_003310)	709930..710304	+	375	WP_226373417.1	protein YgfX	Toxin
IG609_RS03320 (IG609_003315)	710373..711308	+	936	WP_193397385.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
IG609_RS03325 (IG609_003320)	711390..711908	-	519	WP_193397384.1	flavodoxin FldB	-
IG609_RS03330 (IG609_003325)	712071..713399	-	1329	WP_014914180.1	MFS transporter	-
IG609_RS03335 (IG609_003330)	713711..714610	+	900	WP_193397383.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-119)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14626.32 Da
• Isoelectric Point: 10.4737

>T182248 WP_226373417.1 NZ_CP065177:709930-710304 [Pectobacterium quasiaquaticum]
MQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQKNIKSRQGEIVLLSETTLNWRQQEWQIVKRPWLLKN
GVLLSLQAVNGKDKQQLWLASDSMGDDEWRHLRQILLQQKHWAR

Nucleotide

• Length: 375 bp

>T182248 NZ_CP086369:c3054340-3053978 [Pectobacterium versatile]
ATGAGAGTCATCTCAGCCAAGGCCATCACCGATGCGATGAAAAAGCATGCGAAATGGAAAGTTGGTTTGAAGTTATGGCT
GGATGTTTTTGACAAGCCATCGCTGCGGTTTGAGTCCTACGAACAGTTATGTCATGTATGGCGAAATACATCTTGCTGGA
ATGTCGATCGCATACCTTTCGGATTGCTAGAAAACGAAAGCCGCAAAGGACCACTTGACATATATGTATTTGATATTAAT
GGTAACAATTGCAGAATCATAGCATGGTTAAACCCTAATACGGGTTCTTTGTATGTGAAAGACGTGTGCTCTCACGCCGA
GTACGATAAGTGGTGGAAAGCTCAAACGAAGACAAAAAGGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10507.94 Da
• Isoelectric Point: 5.1392

>AT182248 WP_005971623.1 NZ_CP065177:709644-709910 [Pectobacterium quasiaquaticum]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDTDKRTFVRLLQSDDPDLFNWLMNHGEPEDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT182248 NZ_CP086369:c3053974-3053501 [Pectobacterium versatile]
ATGCGAAATACATCTCTGGGTTCTAACAGTTCTGTAGGAATCAGTGGGCTCATCGAAAATTTACCTCTTCTGCTTCAGGA
AGCCTCTGAGCAGCTTAACAGCCTGTCTGTTTTACTCTCTGCCTGTATGGAACCTATTGAAGACGATAATGATCTTCAGG
AAAGAATGGAATGCATCAATCAACTTTACCTCTATTCCTCTGATGTCAATGATATTCCAGCCACTTTTGCAGAGCTCATC
GCGGACAGAGTCTATGAATATGAAAAGAAACACCGAGATGTGCCCCACGTCAGTCAGGCTGAAGCCTTAGCTTTTTTTAT
TGAAGAAAGAGGGCTGAAACAGTCAGATTTAAAGCAAATTGCAACGCAAAGCGTCATCTCTGACATTATTAACGGTAAAC
GAACCATGACATTACAGCAAGTTAAGGATTTTTCTCGCTACTTTAATGTGCCTGTTGAGACATTTATTGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A1D7Z1Z6