Detailed information of TA system TA182243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 26270..26795
• Replicon: plasmid unnamed4
• Accession: NZ_CP065176
• Organism: Klebsiella pneumoniae strain KPN110

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: W8UQV6
• Locus tag: I3U25_RS28735
• Protein ID: WP_001568026.1
• Coordinates: 26490..26795 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: I3U25_RS28730
• Protein ID: WP_004197642.1
• Coordinates: 26270..26488 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U25_RS28710	23032..23424	-	393	Protein_22	IS630 family transposase	-
I3U25_RS28715	23458..24474	+	1017	WP_001310555.1	IS5-like element IS5 family transposase	-
I3U25_RS28720	24705..25676	+	972	WP_099743471.1	sensor domain-containing diguanylate cyclase	-
I3U25_RS28725	25855..26100	+	246	Protein_25	hypothetical protein	-
I3U25_RS28730	26270..26488	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
I3U25_RS28735	26490..26795	+	306	WP_001568026.1	type II toxin-antitoxin system toxin CcdB	Toxin
I3U25_RS28740	26964..27359	+	396	WP_017899885.1	hypothetical protein	-
I3U25_RS28745	27386..27709	+	324	WP_022644730.1	hypothetical protein	-
I3U25_RS28750	27706..28722	+	1017	WP_020315256.1	hypothetical protein	-
I3U25_RS28755	28920..29714	+	795	WP_004197635.1	site-specific integrase	-
I3U25_RS28760	30178..30357	-	180	WP_006788217.1	hypothetical protein	-
I3U25_RS28765	30477..31103	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..101966	101966
	-	flank	IS/Tn	-	-	23494..24474	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11645.32 Da
• Isoelectric Point: 5.6919

>T182243 WP_001568026.1 NZ_CP065176:26490-26795 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T182243 NZ_CP086369:2361928-2362320 [Pectobacterium versatile]
ATGAACTGGGTTAGCGCACAAGAGGTGATCGCTTTTCATGATCGGATATTGCGACATTTACCCGGCGTAGCGGGAATGCC
CGATCCGGGTCGAGCAGAAGCGATAATCTACCGGGTGCAGAATCGTACGCATTATGAAGGAATAACAGACGTCTTCGAAC
TGGCCGCTATTTACTGGGTGGCAGTTGCACGTGGCCATATTTTTAATGATGGCAATAAGCGTACCGCGTTCTTCGTTACG
ATGACGTTTCTATACCGTAACGGCGTATTGATTCGCGATAACGACAACACGTTGGAGAATCTGACGGTAGAGGCTGCAAC
GGGGGCAAAAAACGTTGACCAGTTAGCACAGCATCTACGAAATTTGGCAGACGAGATTAGCCCTACTCGATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT182243 WP_004197642.1 NZ_CP065176:26270-26488 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT182243 NZ_CP086369:2361710-2361931 [Pectobacterium versatile]
ATGCGAACCTACACCTCGACTCAAGCCAGAGCCAACATTTCAGAAGTATTAGATGCTGCAACCCACGGCGAGCCGGTCGA
AATTACTCGTCGAGATGGAAGCTCAGCGGTGGTAATCAGCAAAGCGGAATTCGAGGCATACCAGAATGCCAAATTGGACG
CAGAGTTTGATCTGATTATGCAGCGCCACGGCCACACAATCGAAGCGCTGACTGACCGATGA

Structures

Toxin

• AlphaFold DB: A0A514EZN1