Detailed information of TA system TA182242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 88799..89709
• Replicon: plasmid unnamed2
• Accession: NZ_CP065174
• Organism: Klebsiella pneumoniae strain KPN110

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: I3U25_RS27145
• Protein ID: WP_004026354.1
• Coordinates: 88799..89269 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: I3U25_RS27150
• Protein ID: WP_099745242.1
• Coordinates: 89266..89709 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U25_RS27110	84167..84463	-	297	WP_099745245.1	hydrogenase expression/formation protein HypD	-
I3U25_RS27115	84790..85167	+	378	WP_099745244.1	phosphonate ABC transporter substrate-binding protein	-
I3U25_RS27120	85365..86339	-	975	WP_196241043.1	Hok/Gef family protein	-
I3U25_RS27125	86728..86895	+	168	WP_196241044.1	hypothetical protein	-
I3U25_RS27130	86925..87167	+	243	WP_086528906.1	hypothetical protein	-
I3U25_RS27135	87546..87704	-	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
I3U25_RS27140	88428..88688	+	261	WP_004026352.1	hypothetical protein	-
I3U25_RS27145	88799..89269	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
I3U25_RS27150	89266..89709	-	444	WP_099745242.1	DUF2384 domain-containing protein	Antitoxin
I3U25_RS27155	89810..91081	-	1272	WP_099745241.1	translesion error-prone DNA polymerase V subunit UmuC	-
I3U25_RS27160	91081..91503	-	423	WP_004197884.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
I3U25_RS27165	91857..92189	-	333	Protein_89	transposase	-
I3U25_RS27170	92189..92863	-	675	Protein_90	hypothetical protein	-
I3U25_RS27175	92926..93339	-	414	WP_099745240.1	NB-ARC domain protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..216731	216731
	-	flank	IS/Tn	-	-	93916..94920	1004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T182242 WP_004026354.1 NZ_CP065174:c89269-88799 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T182242 NZ_CP086369:c2359719-2358832 [Pectobacterium versatile]
ATGCTGAAGCAACAAATTCGCCAAATTGTCCAGTCAAACCCTGACTATGCCGCAGTGACACCCGTCATAGAAAAAGAGAT
CTTGCATCATGACATTATCGATGTGCTGATCAAACAGGGCGTCATGCAGCGGTTAACGTTTATTGGCGGCACCTCATTAC
GAATGTGTTACAACAGTAGCAGGCTATCAGAAGATCTTGATTTCAATGGTGGTCATGATTTCCAGCCCTCAGCGTTTGAG
GGGCTGGAGTCCGAAATCCAAACCTATATTCAGAACAAGTATGAAACCGAAGTTTGGGTAAACAAACCTTCTGGCGAGCA
GCAAGGGGATACTGTTTCTTGGAAAATTAGTATCGTAAAAGAAGCTAATCGTCCTGATTTGCCACGGCAAAAAACGCATA
TTGACGTCTGTGCTATTCCGTCATTTGATATTGAAAAACGCCCACTAATCAATCATTACAACATCGTTGTACCGACTGAA
GGGATCTTGGTTCCGGTACAGTCGTTACAAGAAACATTGGCCGATAAGTTGATCGCGTTAGCGTATCGGGCAAGACGTAT
TAAACCTCGTGATATCTGGGATATTGTTTGGATTAAGCAGCGTGGTATTAATTTATCCAAAACACTGGTTGAAAAGAAGC
TCACCGCCAGACAGAAGCAAACAGAAGACTTTCGACAGGCGCTGTCTATTCAGTTAGCCAAGCTAATGTCGGAAGATGAA
GTCCGTGACGACTTCAATATGGAAATGAGCCGCTTTATTCCTAAGCAAATTAAAGAGAGAACCCTCGATAATCCTGAATA
TTGGGCTTATGTTCAGGGGGAGGTACATGCCATCGCGTCTGAGTTACTGAATGACGGAACACCAAAAAATCCATTTAATA
TGGGTTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16494.83 Da
• Isoelectric Point: 10.2498

>AT182242 WP_099745242.1 NZ_CP065174:c89709-89266 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPAELMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT182242 NZ_CP086369:c2360272-2359748 [Pectobacterium versatile]
ATGGCTAAATCTGATGAGCTATTAAGCGCGTTGGCTAAATCAGTGAACGCTGGCGGTGGCATTCACAGTGTAGCAGAGCT
GGCTTTTATGCAGGGTGTGCCTTGTGACTCAGCCTTTAGAAAGTTCCTGGCAGATTGCGTAAAGAAGGGGTTGCTAAGAC
GAGTAGTGAAAGGCTTCTATGAAAGTGTTATTACACCACCTGAGCCTGAGACTGCCATTTATAAAATCATCAAAAAGTTG
CGCAGTGGCGTATTGAACTACATCAGTTTAGAAAGTCAGCTCAGTCATACTGGTGATATTTCACAAGTTGTGATGGGTAG
AGTCACAGTGGTGACTAAAGGTCGAAGTGGTTGTTTTGATACGCCTTATGGCGTGATCGAGTTTACCCATACTAAAAAGC
CAGTCGAGCAAATTGCACCTAATTTGTACTATGACCCCGACATCAAAATGTATCGCGCATACAAGGAACAAGCGATTGCA
GATCTAAAGCACTGCCAGCGCAATCTACATATGTTGGAGAGCTAA

Structures

Toxin

• AlphaFold DB: A0A663AYG0