Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5197780..5198405 Replicon chromosome
Accession NZ_CP065172
Organism Klebsiella pneumoniae strain KPN110

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag I3U25_RS25200 Protein ID WP_021312635.1
Coordinates 5197780..5198163 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I3U25_RS25205 Protein ID WP_004150355.1
Coordinates 5198163..5198405 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U25_RS25185 5195146..5196048 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I3U25_RS25190 5196045..5196680 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I3U25_RS25195 5196677..5197606 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I3U25_RS25200 5197780..5198163 - 384 WP_021312635.1 type II toxin-antitoxin system VapC family toxin Toxin
I3U25_RS25205 5198163..5198405 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I3U25_RS25210 5198610..5199527 + 918 WP_004178029.1 alpha/beta hydrolase -
I3U25_RS25215 5199541..5200482 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I3U25_RS25220 5200527..5200964 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I3U25_RS25225 5200961..5201821 - 861 WP_004146232.1 virulence factor BrkB family protein -
I3U25_RS25230 5201815..5202414 - 600 WP_040148118.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14349.56 Da        Isoelectric Point: 7.3178

>T182240 WP_021312635.1 NZ_CP065172:c5198163-5197780 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVATPYEIHPE

Download         Length: 384 bp

>T182240 NZ_CP086368:c3127021-3125681 [Bordetella pertussis CS]
ATGGGGCGCCGATCGCACAGCCGCAGCCTGTCCATCTGGACCAACGGCGTTCGCGTCGGGCGTTGGACGGTTCCCGCTCG
CGGAGAGATGGAACTGCAGTACGACGTGGATTGGGTCCGTGCCGACGTCGGCCGGCCACTTTCGCTGTCGCTGCCATTCA
ACTTGCAGAATCTGCCCCTCAAGGGGGAGAGAGTCGCCAACTACTTCGACAATCTGCTACCGGACAGCGACGCTATCCGT
CGTCGCGTGGCCGAGCGATTCAGGACGGGCTCGGTCGATCCCTTCGATCTGTTGAAAGCCATTGGACGCGATTGCGTTGG
CGCCGTACAGATCCTGGAAGACGAAGAGATACCTGAGGGCTTTGATCGCATCGAGGGCGTGCCTCTGTCCGAGGAGGACA
TCGAGCGGCATTTGATCGAAACCGTTACGCCGCGCGCCTTCGCTGCGGGCCGGGACCCGGACGCGGATTTCAGAATTTCT
CTGGCCGGCGCGCAAGAGAAGACAGCCTTCCTGTGGTGGGGCGGACAGTGGCTTGCGCCGCGTGGCGCGACATCGACGAG
CCATATTTTCAAACTTCCATTGGGCTTGGTGGGTGGACGGCGGGCTGACTTCAGCACTTCTGTTGATAACGAATGGCTGT
GTCTGAAGCTGCTGAAGGCGTATGGGTTGGATGTGGCTGAGGCCAGCATCGCAACCTTCGGACAGCAGCGAGTGCTGGTG
GTCGAGCGATTCGACCGGCGTGTTGCGCCCAACGGGCAATGGCTGATGCGCCTGCCGCAGGAAGATTTCTGCCAGGTCGA
GGGGTGCTCGCCGCTACGCAAGTATGAAAACGAGGGCGGCCCAGGACTCAAGGCCTTATTCGCCACGCTGCGGCAGTCAG
TGAACGCAGCGGTCGATATGAGAACGTTGATGGCGGCGCAGATCCTGTTTTGGTTGCTGCGGGCACCCGATGGCCACGCC
AAGAATTTCAGCATCCAGTTGTTGCCGCGCGGCCGTTTCCAGTTGACTAGACTGTACGACGTGATGTCGGCCTATCCGGT
ACTGGGCGATGGCGCGAATCAGTGGTCGCCGTTCGAAATCAAATTGGCGATGGCATTGATTGGCAAGAACCGGCATTACG
AGATGCACGGCATCCAGCGCAGGCACTTCAACAGCACCGCACAAAAAGTTGGTTATGGCCCGACGGCGGAGCCGATCATT
GAAGACCTGTTGGCCCGTACGCCCGCGATCATTGCCGCCGTGCAGGCCGATTTGCCGCAGGACTTCTCGCCGCGTGTTGC
GGACTCCGTCCTGGGTGGGCTTGCGCAGGCCGCAAAGTCGCTCGGCGCAATGATGCCGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT182240 WP_004150355.1 NZ_CP065172:c5198405-5198163 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT182240 NZ_CP086368:c3127280-3127023 [Bordetella pertussis CS]
ATGCCGCCGCCATCACAACCTCTGCTCACTACCGGCCAGCTTGGCGCGGTCTTGCAGGCTGCACGCAAAGCACAGGGACT
GACTCAATCGGCCCTGGCCAGTCGTATCGGCCTGAGCCAATCGCGGGTCTCGCACCTGGAGTTGAATGCCCATCAACTGA
GCGTGGAGCAGCTCCTGGCCTGGTGCGCGGCGCTAGGGTTGGAGTTGACGATTGCGACTCGCGGAAGTCCCGCAGGGTCT
TCCGACGCGGATTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References