Detailed information of TA system TA182237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4616211..4617021
• Replicon: chromosome
• Accession: NZ_CP065172
• Organism: Klebsiella pneumoniae strain KPN110

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: I3U25_RS22485
• Protein ID: WP_040148415.1
• Coordinates: 4616211..4616744 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: I3U25_RS22490
• Protein ID: WP_002887278.1
• Coordinates: 4616755..4617021 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U25_RS22480	4615042..4616163	+	1122	WP_016946712.1	cupin domain-containing protein	-
I3U25_RS22485	4616211..4616744	-	534	WP_040148415.1	GNAT family N-acetyltransferase	Toxin
I3U25_RS22490	4616755..4617021	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
I3U25_RS22495	4617124..4618557	-	1434	WP_040148413.1	Cu(+)/Ag(+) sensor histidine kinase	-
I3U25_RS22500	4618547..4619230	-	684	WP_020804437.1	copper response regulator transcription factor CusR	-
I3U25_RS22505	4619402..4620787	+	1386	WP_040148411.1	efflux transporter outer membrane subunit	-
I3U25_RS22510	4620805..4621149	+	345	WP_040148409.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19909.78 Da
• Isoelectric Point: 5.7068

>T182237 WP_040148415.1 NZ_CP065172:c4616744-4616211 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSRDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T182237 NZ_CP086350:c2786251-2785961 [Clavibacter michiganensis subsp. nebraskensis]
GTGAAGCCGCACCGGCTCACGCCGGCCGCGACGAAGGACCTCTCCGAGATCTGGGACTACACGGAGGAGCGCTGGGACGC
CGATCAGGCTGAGACCTATGTCCGGGAGATCGGCGCCGCGATCAGGCGGGTCGCCGAAGACCCCACGCGCGGCCGTGACT
GCGGCGACATCCGCCCGGGCTACCGGCGGTACAGCATCGAGAGCCACACCGTGTACTACGTGGACCGACCCGAGACGGTC
GACGTGATCCGGATCCTGCATCAGCGGATGGACCCGTCGCGCCATCTGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT182237 WP_002887278.1 NZ_CP065172:c4617021-4616755 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT182237 NZ_CP086350:c2786481-2786248 [Clavibacter michiganensis subsp. nebraskensis]
ATGGCGCAGAACACCTCGATCAGCCTGGACGGGCACTTCACCGGCTTCCTCGCCCGGGAGGTGGAGTCCGGGCGCTACCG
ATCCGCGAGCGAGGTGGTCCGCGCCGGGCTCCGTCTCCTGGAGGACCAGGAGACGCAGCTCGAAGCGCTGCGTGCGGCGC
TGGTCGCCGGCGAGGCGAGCGGCGAGGCCGCACCGTTCGACCTCGACGCGTTCATCGCGGACAAGCGCGCGTGA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1