Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4015827..4016446 Replicon chromosome
Accession NZ_CP065172
Organism Klebsiella pneumoniae strain KPN110

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I3U25_RS19640 Protein ID WP_002892050.1
Coordinates 4016228..4016446 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag I3U25_RS19635 Protein ID WP_002892066.1
Coordinates 4015827..4016201 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U25_RS19625 4010979..4012172 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I3U25_RS19630 4012195..4015341 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
I3U25_RS19635 4015827..4016201 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
I3U25_RS19640 4016228..4016446 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
I3U25_RS19645 4016605..4017171 + 567 WP_002892030.1 maltose O-acetyltransferase -
I3U25_RS19650 4017143..4017283 - 141 WP_004147370.1 hypothetical protein -
I3U25_RS19655 4017304..4017774 + 471 WP_002892026.1 YlaC family protein -
I3U25_RS19660 4017749..4019200 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
I3U25_RS19665 4019301..4019999 + 699 WP_099743330.1 GNAT family N-acetyltransferase -
I3U25_RS19670 4019996..4020136 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
I3U25_RS19675 4020136..4020399 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T182236 WP_002892050.1 NZ_CP065172:4016228-4016446 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T182236 NZ_CP086349:c2776953-2776663 [Clavibacter michiganensis subsp. nebraskensis]
GTGAAGCCGCACCGGCTCACGCCGGCCGCGACGAAGGACCTCTCCGAGATCTGGGACTACACGGAGGAGCGCTGGGACGC
CGATCAGGCTGAGACCTATGTCCGGGAGATCGGCGCCGCGATCAGGCGGGTCGCCGAAGACCCCACGCGCGGCCGTGACT
GCGGCGACATCCGCCCGGGCTACCGGCGGTACAGCATCGAGAGCCACACCGTGTACTACGTGGACCGACCCGAGACGGTC
GACGTGATCCGGATCCTGCATCAGCGGATGGACCCGTCGCGCCATCTGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT182236 WP_002892066.1 NZ_CP065172:4015827-4016201 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT182236 NZ_CP086349:c2777183-2776950 [Clavibacter michiganensis subsp. nebraskensis]
ATGGCGCAGAACACCTCGATCAGCCTGGACGGGCACTTCACCGGCTTCCTCGCCCGGGAGGTGGAGTCCGGGCGCTACCG
ATCCGCGAGCGAGGTGGTCCGCGCCGGGCTCCGTCTCCTGGAGGACCAGGAGACGCAGCTCGAAGCGCTGCGTGCGGCGC
TGGTCGCCGGCGAGGCGAGCGGCGAGGCCGCACCGTTCGACCTCGACGCGTTCATCGCGGACAAGCGCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References