Detailed information of TA system TA182235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2649971..2651559
• Replicon: chromosome
• Accession: NZ_CP065172
• Organism: Klebsiella pneumoniae strain KPN110

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: I3U25_RS13005
• Protein ID: WP_040148030.1
• Coordinates: 2649971..2651293 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: I3U25_RS13010
• Protein ID: WP_002904790.1
• Coordinates: 2651293..2651559 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U25_RS12990	2645027..2647528	+	2502	WP_040148028.1	fimbrial biogenesis outer membrane usher protein	-
I3U25_RS12995	2647516..2648520	+	1005	WP_040148029.1	type 1 fimbrial protein	-
I3U25_RS13000	2648662..2649954	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
I3U25_RS13005	2649971..2651293	-	1323	WP_040148030.1	type II toxin-antitoxin system HipA family toxin	Toxin
I3U25_RS13010	2651293..2651559	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I3U25_RS13015	2651824..2652150	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
I3U25_RS13020	2652147..2652647	-	501	WP_040148032.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
I3U25_RS13025	2652625..2654004	-	1380	WP_004184021.1	purine permease	-
I3U25_RS13030	2654350..2655504	+	1155	WP_040148035.1	FAD-dependent urate hydroxylase HpxO	-
I3U25_RS13035	2655485..2656408	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5366

>T182235 WP_040148030.1 NZ_CP065172:c2651293-2649971 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T182235 NZ_CP086348:c2777127-2776837 [Clavibacter michiganensis subsp. nebraskensis]
GTGAAGCCGCACCGGCTCACGCCGGCCGCGACGAAGGACCTCTCCGAGATCTGGGACTACACGGAGGAGCGCTGGGACGC
CGATCAGGCTGAGACCTATGTCCGGGAGATCGGCGCCGCGATCAGGCGGGTCGCCGAAGACCCCACGCGCGGCCGTGACT
GCGGCGACATCCGCCCGGGCTACCGGCGGTACAGCATCGAGAGCCACACCGTGTACTACGTGGACCGACCCGAGACGGTC
GACGTGATCCGGATCCTGCATCAGCGGATGGACCCGTCGCGCCATCTGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT182235 WP_002904790.1 NZ_CP065172:c2651559-2651293 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT182235 NZ_CP086348:c2777357-2777124 [Clavibacter michiganensis subsp. nebraskensis]
ATGGCGCAGAACACCTCGATCAGCCTGGACGGGCACTTCACCGGCTTCCTCGCCCGGGAGGTGGAGTCCGGGCGCTACCG
ATCCGCGAGCGAGGTGGTCCGCGCCGGGCTCCGTCTCCTGGAGGACCAGGAGACGCAGCTCGAAGCGCTGCGTGCGGCGC
TGGTCGCCGGCGAGGCGAGCGGCGAGGCCGCACCGTTCGACCTCGACGCGTTCATCGCGGACAAGCGCGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8