Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4657944..4658754 Replicon chromosome
Accession NZ_CP065167
Organism Klebsiella pneumoniae strain KPN114

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag I3U24_RS22860 Protein ID WP_004178461.1
Coordinates 4657944..4658477 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag I3U24_RS22865 Protein ID WP_002887278.1
Coordinates 4658488..4658754 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U24_RS22855 4656775..4657896 + 1122 WP_021441121.1 cupin domain-containing protein -
I3U24_RS22860 4657944..4658477 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
I3U24_RS22865 4658488..4658754 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
I3U24_RS22870 4658857..4660290 - 1434 WP_021441120.1 Cu(+)/Ag(+) sensor histidine kinase -
I3U24_RS22875 4660280..4660963 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
I3U24_RS22880 4661136..4662521 + 1386 WP_038806759.1 efflux transporter outer membrane subunit -
I3U24_RS22885 4662539..4662883 + 345 WP_021441118.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T182218 WP_004178461.1 NZ_CP065167:c4658477-4657944 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T182218 NZ_CP086342:c2119325-2119218 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT182218 WP_002887278.1 NZ_CP065167:c4658754-4658488 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT182218 NZ_CP086342:2119372-2119438 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References