Detailed information of TA system TA182216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2641340..2642928
• Replicon: chromosome
• Accession: NZ_CP065167
• Organism: Klebsiella pneumoniae strain KPN114

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A6T928
• Locus tag: I3U24_RS13015
• Protein ID: WP_004176197.1
• Coordinates: 2641340..2642662 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I3U24_RS13020
• Protein ID: WP_032417111.1
• Coordinates: 2642662..2642928 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U24_RS13000	2636396..2638897	+	2502	WP_016831617.1	fimbrial biogenesis outer membrane usher protein	-
I3U24_RS13005	2638885..2639889	+	1005	WP_004176196.1	type 1 fimbrial protein	-
I3U24_RS13010	2640031..2641323	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
I3U24_RS13015	2641340..2642662	-	1323	WP_004176197.1	type II toxin-antitoxin system HipA family toxin	Toxin
I3U24_RS13020	2642662..2642928	-	267	WP_032417111.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I3U24_RS13025	2643193..2643519	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
I3U24_RS13030	2643516..2644016	-	501	WP_002904787.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
I3U24_RS13035	2643994..2645373	-	1380	WP_032429500.1	purine permease	-
I3U24_RS13040	2645719..2646873	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
I3U24_RS13045	2646854..2647777	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2442571..2676595	234024

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49111.41 Da
• Isoelectric Point: 7.5357

>T182216 WP_004176197.1 NZ_CP065167:c2642662-2641340 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T182216 NZ_CP086342:c1302851-1302633 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9926.32 Da
• Isoelectric Point: 8.5912

>AT182216 WP_032417111.1 NZ_CP065167:c2642928-2642662 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETASAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT182216 NZ_CP086342:c1303251-1302877 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A6T928