Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 736950..737707 Replicon chromosome
Accession NZ_CP065167
Organism Klebsiella pneumoniae strain KPN114

Toxin (Protein)


Gene name tacT Uniprot ID A0A1D8JMF4
Locus tag I3U24_RS03650 Protein ID WP_044159004.1
Coordinates 737225..737707 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A1D8JMH3
Locus tag I3U24_RS03645 Protein ID WP_007372584.1
Coordinates 736950..737234 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U24_RS03625 732342..733064 + 723 WP_013095151.1 TIGR03761 family integrating conjugative element protein -
I3U24_RS03630 733082..735094 + 2013 WP_044159002.1 DNA topoisomerase III -
I3U24_RS03635 735727..736218 + 492 WP_007372582.1 DUF3577 domain-containing protein -
I3U24_RS03640 736294..736794 + 501 WP_007372583.1 single-stranded DNA-binding protein -
I3U24_RS03645 736950..737234 + 285 WP_007372584.1 DUF1778 domain-containing protein Antitoxin
I3U24_RS03650 737225..737707 + 483 WP_044159004.1 GNAT family N-acetyltransferase Toxin
I3U24_RS03655 737940..739226 + 1287 WP_013095156.1 TcpQ domain-containing protein -
I3U24_RS03660 739228..739671 + 444 WP_007372587.1 type IV pilus biogenesis protein PilM -
I3U24_RS03665 739691..741364 + 1674 WP_013095157.1 PilN family type IVB pilus formation outer membrane protein -
I3U24_RS03670 741367..742647 + 1281 WP_176682058.1 type 4b pilus protein PilO2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 724820..832256 107436


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(39-146)

Antitoxin

Vibrio phage ICP1, Orf50

(1-93)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17693.32 Da        Isoelectric Point: 6.9570

>T182206 WP_044159004.1 NZ_CP065167:737225-737707 [Klebsiella pneumoniae]
MEINVTAPALLTEEHVLHSFDCGNAVLDDWLRRRALKNQHLNASRTFVICPEGTACVVGYYSLATGSVSHVDLSRSLRQN
MPDPVPVVLLGRLAVDVSTQGNNFGKWLLNDAVMRVSNLADQVGIKAIMVHAIDERAKAFYEYFGFVQSPVAANTLFYKI

Download         Length: 483 bp

>T182206 NZ_CP086340:c3751210-3750812 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 11078.63 Da        Isoelectric Point: 8.5684

>AT182206 WP_007372584.1 NZ_CP065167:736950-737234 [Klebsiella pneumoniae]
MQTPVRKSVRDKQINIRATDEERAVIDYAASLVNKNRTDFIIERAVHEAQNIILDQRVFVLDDARYQAFIRQLEAPVQNV
EGRQRLMDVKPEWK

Download         Length: 285 bp

>AT182206 NZ_CP086340:c3751506-3751213 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8JMF4

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JMH3

References