Detailed information of TA system TA182202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53536..54272
• Replicon: plasmid unnamed2
• Accession: NZ_CP065164
• Organism: Klebsiella variicola strain KPN029

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: I3U23_RS29020
• Protein ID: WP_003026803.1
• Coordinates: 53790..54272 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I3U23_RS29015
• Protein ID: WP_003026799.1
• Coordinates: 53536..53802 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U23_RS28970	49736..50098	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
I3U23_RS28975	50148..50498	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
I3U23_RS28980	50856..51104	+	249	WP_019725036.1	hypothetical protein	-
I3U23_RS28985	51101..51673	+	573	WP_015065504.1	hypothetical protein	-
I3U23_RS28990	51704..52198	+	495	WP_009310053.1	hypothetical protein	-
I3U23_RS28995	52242..52610	+	369	WP_015065502.1	hypothetical protein	-
I3U23_RS29000	52644..52847	+	204	WP_019725040.1	hemolysin expression modulator Hha	-
I3U23_RS29005	52861..53091	+	231	WP_019725034.1	hypothetical protein	-
I3U23_RS29010	53135..53329	-	195	WP_019725033.1	hypothetical protein	-
I3U23_RS29015	53536..53802	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I3U23_RS29020	53790..54272	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
I3U23_RS29025	54480..55826	+	1347	WP_077253535.1	ISNCY family transposase	-
I3U23_RS29030	55875..56270	+	396	WP_004143398.1	helix-turn-helix domain-containing protein	-
I3U23_RS29035	56418..57672	-	1255	Protein_58	IS3 family transposase	-
I3U23_RS29040	57760..58722	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 / qnrB1 / tet(A) / aac(6')-Ib-cr / blaOXA-1	-	1..243621	243621

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T182202 WP_003026803.1 NZ_CP065164:53790-54272 [Klebsiella variicola]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T182202 NZ_CP086340:c2433868-2433692 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT182202 WP_003026799.1 NZ_CP065164:53536-53802 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT182202 NZ_CP086340:c2433646-2433230 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66