Detailed information of TA system TA182201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 124599..125509
• Replicon: plasmid unnamed1
• Accession: NZ_CP065163
• Organism: Klebsiella variicola strain KPN029

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: I3U23_RS28065
• Protein ID: WP_004026354.1
• Coordinates: 124599..125069 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: I3U23_RS28070
• Protein ID: WP_099745242.1
• Coordinates: 125066..125509 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U23_RS28030	119967..120263	-	297	WP_099745245.1	hydrogenase expression/formation protein HypD	-
I3U23_RS28035	120590..120967	+	378	WP_099745244.1	phosphonate ABC transporter substrate-binding protein	-
I3U23_RS28040	121165..122139	-	975	WP_196241043.1	Hok/Gef family protein	-
I3U23_RS28045	122528..122695	+	168	WP_196241044.1	hypothetical protein	-
I3U23_RS28050	122725..122967	+	243	WP_086528906.1	hypothetical protein	-
I3U23_RS28055	123346..123504	-	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
I3U23_RS28060	124228..124488	+	261	WP_004026352.1	hypothetical protein	-
I3U23_RS28065	124599..125069	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
I3U23_RS28070	125066..125509	-	444	WP_099745242.1	DUF2384 domain-containing protein	Antitoxin
I3U23_RS28075	125610..126881	-	1272	WP_099745241.1	translesion error-prone DNA polymerase V subunit UmuC	-
I3U23_RS28080	126881..127303	-	423	WP_004197884.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
I3U23_RS28085	127388..128875	-	1488	WP_171996283.1	group II intron reverse transcriptase/maturase	-
I3U23_RS28090	129567..129899	-	333	Protein_140	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..250975	250975

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T182201 WP_004026354.1 NZ_CP065163:c125069-124599 [Klebsiella variicola]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T182201 NZ_CP086340:2346709-2348031 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16494.83 Da
• Isoelectric Point: 10.2498

>AT182201 WP_099745242.1 NZ_CP065163:c125509-125066 [Klebsiella variicola]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPAELMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT182201 NZ_CP086340:2346443-2346709 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A663AYG0