Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5495165..5495790 Replicon chromosome
Accession NZ_CP065162
Organism Klebsiella variicola strain KPN029

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag I3U23_RS27025 Protein ID WP_008807903.1
Coordinates 5495165..5495548 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I3U23_RS27030 Protein ID WP_004150355.1
Coordinates 5495548..5495790 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U23_RS27010 5492531..5493433 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I3U23_RS27015 5493430..5494065 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
I3U23_RS27020 5494062..5494991 + 930 WP_055323209.1 formate dehydrogenase accessory protein FdhE -
I3U23_RS27025 5495165..5495548 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
I3U23_RS27030 5495548..5495790 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I3U23_RS27035 5495995..5496912 + 918 WP_032733990.1 alpha/beta hydrolase -
I3U23_RS27040 5496927..5497868 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
I3U23_RS27045 5497913..5498350 - 438 WP_012543288.1 D-tyrosyl-tRNA(Tyr) deacylase -
I3U23_RS27050 5498347..5499207 - 861 WP_008807907.1 virulence factor BrkB family protein -
I3U23_RS27055 5499201..5499800 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T182200 WP_008807903.1 NZ_CP065162:c5495548-5495165 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T182200 NZ_CP086340:2036079-2036182 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT182200 WP_004150355.1 NZ_CP065162:c5495790-5495548 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT182200 NZ_CP086340:c2036220-2035949 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References