Detailed information of TA system TA182199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5014688..5015204
• Replicon: chromosome
• Accession: NZ_CP065162
• Organism: Klebsiella variicola strain KPN029

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: I3U23_RS24745
• Protein ID: WP_022065401.1
• Coordinates: 5014688..5014972 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R4Y888
• Locus tag: I3U23_RS24750
• Protein ID: WP_002886901.1
• Coordinates: 5014962..5015204 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U23_RS24730	5009791..5011446	+	1656	WP_008807132.1	alpha,alpha-phosphotrehalase	-
I3U23_RS24735	5011832..5013970	+	2139	WP_032731807.1	anaerobic ribonucleoside-triphosphate reductase	-
I3U23_RS24740	5014220..5014684	+	465	WP_022065402.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
I3U23_RS24745	5014688..5014972	-	285	WP_022065401.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I3U23_RS24750	5014962..5015204	-	243	WP_002886901.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
I3U23_RS24755	5015282..5017192	-	1911	WP_032756354.1	BglG family transcription antiterminator	-
I3U23_RS24760	5017215..5018369	-	1155	WP_032756352.1	lactonase family protein	-
I3U23_RS24765	5018495..5019235	-	741	WP_008807137.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11128.97 Da
• Isoelectric Point: 10.2849

>T182199 WP_022065401.1 NZ_CP065162:c5014972-5014688 [Klebsiella variicola]
MTYELEFDPRAWREWQMPGETVKKQFKNKLQQIVQNPRIESARLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T182199 NZ_CP086340:c1872550-1872176 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9045.55 Da
• Isoelectric Point: 6.3340

>AT182199 WP_002886901.1 NZ_CP065162:c5015204-5014962 [Klebsiella variicola]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT182199 NZ_CP086340:c1873008-1872640 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GLP0