Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4313743..4314362 Replicon chromosome
Accession NZ_CP065162
Organism Klebsiella variicola strain KPN029

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I3U23_RS21440 Protein ID WP_002892050.1
Coordinates 4314144..4314362 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag I3U23_RS21435 Protein ID WP_008805436.1
Coordinates 4313743..4314117 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U23_RS21425 4308898..4310091 + 1194 WP_012542667.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I3U23_RS21430 4310114..4313260 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
I3U23_RS21435 4313743..4314117 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
I3U23_RS21440 4314144..4314362 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
I3U23_RS21445 4314521..4315087 + 567 WP_008805435.1 maltose O-acetyltransferase -
I3U23_RS21450 4315059..4315169 - 111 Protein_4215 hypothetical protein -
I3U23_RS21455 4315224..4315694 + 471 WP_008805434.1 YlaC family protein -
I3U23_RS21460 4315663..4317120 - 1458 WP_008805433.1 PLP-dependent aminotransferase family protein -
I3U23_RS21465 4317221..4317919 + 699 WP_023297138.1 GNAT family N-acetyltransferase -
I3U23_RS21470 4317916..4318056 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
I3U23_RS21475 4318056..4318319 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T182198 WP_002892050.1 NZ_CP065162:4314144-4314362 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T182198 NZ_CP086340:1855703-1855957 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT182198 WP_008805436.1 NZ_CP065162:4313743-4314117 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT182198 NZ_CP086340:1855455-1855706 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References