Detailed information of TA system TA182196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2782030..2783618
• Replicon: chromosome
• Accession: NZ_CP065162
• Organism: Klebsiella variicola strain KPN029

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A1F2MCM0
• Locus tag: I3U23_RS13835
• Protein ID: WP_008804919.1
• Coordinates: 2782030..2783352 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1F2MCN9
• Locus tag: I3U23_RS13840
• Protein ID: WP_008804920.1
• Coordinates: 2783352..2783618 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U23_RS13820	2777095..2779596	+	2502	WP_137963366.1	fimbrial biogenesis outer membrane usher protein	-
I3U23_RS13825	2779584..2780588	+	1005	WP_117106511.1	type 1 fimbrial protein	-
I3U23_RS13830	2780721..2782013	+	1293	WP_012968243.1	glycoside hydrolase family 10 protein	-
I3U23_RS13835	2782030..2783352	-	1323	WP_008804919.1	type II toxin-antitoxin system HipA family toxin	Toxin
I3U23_RS13840	2783352..2783618	-	267	WP_008804920.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I3U23_RS13845	2783884..2784210	-	327	WP_008804921.1	hydroxyisourate hydrolase	-
I3U23_RS13850	2784207..2784707	-	501	WP_049153027.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
I3U23_RS13855	2784685..2786064	-	1380	WP_012968246.1	purine permease	-
I3U23_RS13860	2786410..2787564	+	1155	WP_008804924.1	FAD-dependent urate hydroxylase HpxO	-
I3U23_RS13865	2787545..2788468	-	924	WP_008804925.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 48911.26 Da
• Isoelectric Point: 7.9109

>T182196 WP_008804919.1 NZ_CP065162:c2783352-2782030 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGEQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T182196 NZ_CP086340:1075722-1076057 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.30 Da
• Isoelectric Point: 8.5912

>AT182196 WP_008804920.1 NZ_CP065162:c2783618-2783352 [Klebsiella variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT182196 NZ_CP086340:1075474-1075722 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A1F2MCM0

Antitoxin

• AlphaFold DB: A0A1F2MCN9