Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 777341..777998 Replicon chromosome
Accession NZ_CP065162
Organism Klebsiella variicola strain KPN029

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag I3U23_RS03850 Protein ID WP_002916310.1
Coordinates 777588..777998 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag I3U23_RS03845 Protein ID WP_002916312.1
Coordinates 777341..777607 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U23_RS03820 772490..773923 - 1434 WP_032740988.1 6-phospho-beta-glucosidase BglA -
I3U23_RS03825 774043..774771 - 729 WP_012967245.1 MurR/RpiR family transcriptional regulator -
I3U23_RS03830 774822..775133 + 312 WP_008806430.1 N(4)-acetylcytidine aminohydrolase -
I3U23_RS03835 775297..775956 + 660 WP_008806429.1 hemolysin III family protein -
I3U23_RS03840 776112..777095 - 984 WP_008806428.1 tRNA-modifying protein YgfZ -
I3U23_RS03845 777341..777607 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
I3U23_RS03850 777588..777998 + 411 WP_002916310.1 protein YgfX Toxin
I3U23_RS03855 778005..778526 - 522 WP_008806427.1 flavodoxin FldB -
I3U23_RS03860 778627..779523 + 897 WP_008806426.1 site-specific tyrosine recombinase XerD -
I3U23_RS03865 779546..780259 + 714 WP_032736403.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I3U23_RS03870 780265..781998 + 1734 WP_012540594.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T182186 WP_002916310.1 NZ_CP065162:777588-777998 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T182186 NZ_CP086338:c3871180-3870884 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT182186 WP_002916312.1 NZ_CP065162:777341-777607 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT182186 NZ_CP086338:c3870882-3870487 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References