Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 710608..711383 Replicon chromosome
Accession NZ_CP065162
Organism Klebsiella variicola strain KPN029

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A2N4Z6P0
Locus tag I3U23_RS03510 Protein ID WP_032740995.1
Coordinates 710898..711383 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag I3U23_RS03505 Protein ID WP_004150912.1
Coordinates 710608..710901 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U23_RS03485 705865..706467 - 603 WP_086512099.1 short chain dehydrogenase -
I3U23_RS03490 706565..707476 + 912 WP_086512098.1 LysR family transcriptional regulator -
I3U23_RS03495 707477..708625 - 1149 WP_117106709.1 PLP-dependent aspartate aminotransferase family protein -
I3U23_RS03500 708637..710013 - 1377 WP_016161835.1 cystathionine beta-synthase -
I3U23_RS03505 710608..710901 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
I3U23_RS03510 710898..711383 + 486 WP_032740995.1 GNAT family N-acetyltransferase Toxin
I3U23_RS03515 712087..712679 + 593 Protein_690 hypothetical protein -
I3U23_RS03520 712776..712992 + 217 Protein_691 transposase -
I3U23_RS03530 713516..714229 - 714 WP_008806478.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 712776..712928 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17618.64 Da        Isoelectric Point: 8.5178

>T182185 WP_032740995.1 NZ_CP065162:710898-711383 [Klebsiella variicola]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVH
A

Download         Length: 486 bp

>T182185 NZ_CP086338:3795248-3795625 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT182185 WP_004150912.1 NZ_CP065162:710608-710901 [Klebsiella variicola]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT182185 NZ_CP086338:3794824-3795201 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N4Z6P0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References