Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 279011..279642 Replicon chromosome
Accession NZ_CP065162
Organism Klebsiella variicola strain KPN029

Toxin (Protein)


Gene name hicA Uniprot ID A0A2V3KJT3
Locus tag I3U23_RS01260 Protein ID WP_044650325.1
Coordinates 279011..279286 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag I3U23_RS01265 Protein ID WP_004181489.1
Coordinates 279283..279642 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U23_RS01240 274247..274582 + 336 WP_002921203.1 universal stress protein UspB -
I3U23_RS01245 274642..276138 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
I3U23_RS01250 276368..277561 + 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
I3U23_RS01255 277601..278614 - 1014 WP_022066424.1 magnesium transporter -
I3U23_RS01260 279011..279286 + 276 WP_044650325.1 type II toxin-antitoxin system HicA family toxin Toxin
I3U23_RS01265 279283..279642 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
I3U23_RS01270 279670..280071 - 402 WP_023323361.1 nickel-responsive transcriptional regulator NikR -
I3U23_RS01275 280059..280850 - 792 WP_008806945.1 nickel import ATP-binding protein NikE -
I3U23_RS01280 280847..281611 - 765 WP_008806946.1 nickel import ATP-binding protein NikD -
I3U23_RS01285 281611..282444 - 834 WP_012540374.1 nickel ABC transporter permease subunit NikC -
I3U23_RS01290 282441..283385 - 945 WP_008806948.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10279.91 Da        Isoelectric Point: 10.3165

>T182182 WP_044650325.1 NZ_CP065162:279011-279286 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T182182 NZ_CP086338:c2707147-2706896 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT182182 WP_004181489.1 NZ_CP065162:279283-279642 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT182182 NZ_CP086338:c2706899-2706645 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KJT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References