Detailed information of TA system TA182181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3136057..3136974
• Replicon: chromosome
• Accession: NZ_CP065159
• Organism: Bacillus amyloliquefaciens strain GXU-1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: I3J23_RS16225
• Protein ID: WP_025851786.1
• Coordinates: 3136228..3136974 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: I3J23_RS16220
• Protein ID: WP_003154807.1
• Coordinates: 3136057..3136227 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3J23_RS16185	3131288..3132910	+	1623	WP_014304855.1	pyocin knob domain-containing protein	-
I3J23_RS16190	3132923..3133294	+	372	Protein_3121	hypothetical protein	-
I3J23_RS16195	3133300..3133497	+	198	WP_003154819.1	XkdX family protein	-
I3J23_RS16200	3133554..3134315	+	762	WP_043867020.1	hypothetical protein	-
I3J23_RS16205	3134367..3134630	+	264	WP_003154815.1	hemolysin XhlA family protein	-
I3J23_RS16210	3134644..3134907	+	264	WP_003154813.1	phage holin	-
I3J23_RS16215	3134921..3135799	+	879	WP_024085195.1	N-acetylmuramoyl-L-alanine amidase	-
I3J23_RS16220	3136057..3136227	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
I3J23_RS16225	3136228..3136974	-	747	WP_025851786.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
I3J23_RS16230	3137078..3138076	-	999	WP_003154805.1	inorganic phosphate transporter	-
I3J23_RS16235	3138089..3138706	-	618	WP_003154804.1	DUF47 domain-containing protein	-
I3J23_RS16240	3138992..3140308	-	1317	WP_003154801.1	amino acid permease	-
I3J23_RS16245	3140631..3141581	+	951	WP_043867021.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29048.53 Da
• Isoelectric Point: 4.7003

>T182181 WP_025851786.1 NZ_CP065159:c3136974-3136228 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CEYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T182181 NZ_CP086338:c2706899-2706645 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT182181 WP_003154807.1 NZ_CP065159:c3136227-3136057 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT182181 NZ_CP086338:c2707147-2706896 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14