Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2377283..2377920 Replicon chromosome
Accession NZ_CP065159
Organism Bacillus amyloliquefaciens strain GXU-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag I3J23_RS12240 Protein ID WP_003156187.1
Coordinates 2377570..2377920 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag I3J23_RS12235 Protein ID WP_003156188.1
Coordinates 2377283..2377564 (+) Length 94 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3J23_RS12215 2373648..2374247 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
I3J23_RS12220 2374340..2374705 + 366 WP_014304402.1 holo-ACP synthase -
I3J23_RS12225 2374870..2375877 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
I3J23_RS12230 2375994..2377163 + 1170 WP_003156189.1 alanine racemase -
I3J23_RS12235 2377283..2377564 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
I3J23_RS12240 2377570..2377920 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
I3J23_RS12245 2378038..2378859 + 822 WP_003156182.1 STAS domain-containing protein -
I3J23_RS12250 2378864..2379229 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
I3J23_RS12255 2379232..2379633 + 402 WP_003156178.1 anti-sigma regulatory factor -
I3J23_RS12260 2379645..2380652 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
I3J23_RS12265 2380716..2381045 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
I3J23_RS12270 2381042..2381524 + 483 WP_003156173.1 anti-sigma B factor RsbW -
I3J23_RS12275 2381490..2382278 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
I3J23_RS12280 2382278..2382880 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-111)

Antitoxin

(12-45)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T182180 WP_003156187.1 NZ_CP065159:2377570-2377920 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T182180 NZ_CP065159:2377570-2377920 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT182180 WP_003156188.1 NZ_CP065159:2377283-2377564 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT182180 NZ_CP065159:2377283-2377564 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8


Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References