Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | pemIK/MazF(toxin) |
| Location | 2754602..2755253 | Replicon | chromosome |
| Accession | NZ_CP065154 | ||
| Organism | Lacticaseibacillus paracasei subsp. tolerans strain ZY-1 | ||
Toxin (Protein)
| Gene name | pemK | Uniprot ID | K6S8L5 |
| Locus tag | I3F57_RS13335 | Protein ID | WP_003567661.1 |
| Coordinates | 2754602..2754985 (-) | Length | 128 a.a. |
Antitoxin (Protein)
| Gene name | pemI | Uniprot ID | K6RKI5 |
| Locus tag | I3F57_RS13340 | Protein ID | WP_003567665.1 |
| Coordinates | 2755005..2755253 (-) | Length | 83 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| I3F57_RS13330 | 2753897..2754418 | - | 522 | WP_003567657.1 | QueT transporter family protein | - |
| I3F57_RS13335 | 2754602..2754985 | - | 384 | WP_003567661.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| I3F57_RS13340 | 2755005..2755253 | - | 249 | WP_003567665.1 | antitoxin | Antitoxin |
| I3F57_RS13345 | 2755321..2756457 | - | 1137 | WP_003581082.1 | alanine racemase | - |
| I3F57_RS13350 | 2756444..2756818 | - | 375 | WP_003567669.1 | holo-ACP synthase | - |
| I3F57_RS13355 | 2756987..2758495 | - | 1509 | WP_003567670.1 | DEAD/DEAH box helicase | - |
| I3F57_RS13360 | 2758621..2758764 | - | 144 | WP_016380391.1 | hypothetical protein | - |
| I3F57_RS13365 | 2758795..2760183 | - | 1389 | WP_003581096.1 | UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 128 a.a. Molecular weight: 13773.03 Da Isoelectric Point: 10.9764
>T182179 WP_003567661.1 NZ_CP065154:c2754985-2754602 [Lacticaseibacillus paracasei subsp. tolerans]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN
Download Length: 384 bp
>T182179 NZ_CP086338:c2527686-2527583 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT
Antitoxin
Download Length: 83 a.a. Molecular weight: 8938.08 Da Isoelectric Point: 4.4610
>AT182179 WP_003567665.1 NZ_CP065154:c2755253-2755005 [Lacticaseibacillus paracasei subsp. tolerans]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR
Download Length: 249 bp
>AT182179 NZ_CP086338:2527545-2527816 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | K6S8L5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E2BNY2 |