Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 188861..189504 Replicon plasmid unnamed1
Accession NZ_CP065153
Organism Escherichia coli O150:H6 strain FEX669

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag H7994_RS24870 Protein ID WP_001034044.1
Coordinates 189088..189504 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag H7994_RS24865 Protein ID WP_001261286.1
Coordinates 188861..189091 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7994_RS24850 183998..184228 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H7994_RS24855 184225..184641 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
H7994_RS24860 184686..188480 - 3795 WP_170930870.1 pcar -
H7994_RS24865 188861..189091 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7994_RS24870 189088..189504 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
H7994_RS24875 189579..191143 + 1565 Protein_200 AAA family ATPase -
H7994_RS24880 191128..192150 + 1023 WP_000361402.1 helicase UvrD -
H7994_RS24885 192404..193101 - 698 Protein_202 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(6)-Id / aph(3'')-Ib / blaTEM-1B / tet(A) / aph(3')-IIa / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA / iroB / iroC 1..237102 237102
- flank IS/Tn - - 192404..192907 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T182176 WP_001034044.1 NZ_CP065153:189088-189504 [Escherichia coli O150:H6]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T182176 NZ_CP086338:c1917015-1916908 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT182176 WP_001261286.1 NZ_CP065153:188861-189091 [Escherichia coli O150:H6]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT182176 NZ_CP086338:1917062-1917128 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References