Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 183998..184641 Replicon plasmid unnamed1
Accession NZ_CP065153
Organism Escherichia coli O150:H6 strain FEX669

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag H7994_RS24855 Protein ID WP_001034046.1
Coordinates 184225..184641 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag H7994_RS24850 Protein ID WP_001261278.1
Coordinates 183998..184228 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7994_RS24825 179997..180776 - 780 WP_001297096.1 IS21-like element IS100kyp family helper ATPase IstB -
H7994_RS24830 180776..181798 - 1023 WP_001375934.1 IS21-like element IS100 family transposase -
H7994_RS24835 182080..182886 - 807 WP_072650694.1 site-specific integrase -
H7994_RS24840 182887..183192 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
H7994_RS24845 183194..183412 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
H7994_RS24850 183998..184228 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7994_RS24855 184225..184641 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
H7994_RS24860 184686..188480 - 3795 WP_170930870.1 pcar -
H7994_RS24865 188861..189091 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H7994_RS24870 189088..189504 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(6)-Id / aph(3'')-Ib / blaTEM-1B / tet(A) / aph(3')-IIa / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA / iroB / iroC 1..237102 237102


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T182175 WP_001034046.1 NZ_CP065153:184225-184641 [Escherichia coli O150:H6]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T182175 NZ_CP086338:c1916480-1916373 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT182175 WP_001261278.1 NZ_CP065153:183998-184228 [Escherichia coli O150:H6]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT182175 NZ_CP086338:1916527-1916593 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References