Detailed information of TA system TA182169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 131173..131798
• Replicon: plasmid unnamed1
• Accession: NZ_CP065153
• Organism: Escherichia coli O150:H6 strain FEX669

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H7994_RS24540
• Protein ID: WP_000911333.1
• Coordinates: 131400..131798 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: H7994_RS24535
• Protein ID: WP_000450520.1
• Coordinates: 131173..131400 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7994_RS24535	131173..131400	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
H7994_RS24540	131400..131798	+	399	WP_000911333.1	tRNA(fMet)-specific endonuclease VapC	Toxin
H7994_RS24545	131807..133960	-	2154	Protein_134	type IV conjugative transfer system coupling protein TraD	-
H7994_RS24550	134021..134698	+	678	WP_196316085.1	IS66-like element accessory protein TnpA	-
H7994_RS24555	134698..135045	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
H7994_RS24560	135065..136636	+	1572	WP_000381395.1	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / blaTEM-1B / tet(A) / aph(3')-IIa / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA / iroB / iroC	1..237102	237102

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14919.19 Da
• Isoelectric Point: 7.8605

>T182169 WP_000911333.1 NZ_CP065153:131400-131798 [Escherichia coli O150:H6]
MLKFMLDTNICIFTMKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T182169 NZ_CP086338:288435-288608 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT182169 WP_000450520.1 NZ_CP065153:131173-131400 [Escherichia coli O150:H6]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT182169 NZ_CP086338:288111-288407 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: V0VCB2