Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-YafN |
| Location | 91239..91774 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP065153 | ||
| Organism | Escherichia coli O150:H6 strain FEX669 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | V0VJJ7 |
| Locus tag | H7994_RS24350 | Protein ID | WP_000222760.1 |
| Coordinates | 91239..91526 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0VIP2 |
| Locus tag | H7994_RS24355 | Protein ID | WP_001132900.1 |
| Coordinates | 91523..91774 (-) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H7994_RS24325 | 86822..88043 | - | 1222 | Protein_90 | ISL3 family transposase | - |
| H7994_RS24330 | 88143..88507 | + | 365 | Protein_91 | IS1 family transposase | - |
| H7994_RS24335 | 88562..89341 | - | 780 | WP_176541790.1 | IS21-like element IS100kyp family helper ATPase IstB | - |
| H7994_RS24340 | 89341..90363 | - | 1023 | WP_000255956.1 | IS21-like element IS100 family transposase | - |
| H7994_RS24345 | 90452..90799 | + | 348 | Protein_94 | IS1-like element IS1A family transposase | - |
| H7994_RS24350 | 91239..91526 | - | 288 | WP_000222760.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| H7994_RS24355 | 91523..91774 | - | 252 | WP_001132900.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| H7994_RS24360 | 92737..93593 | - | 857 | Protein_97 | incFII family plasmid replication initiator RepA | - |
| H7994_RS24365 | 93586..94068 | - | 483 | WP_001273588.1 | hypothetical protein | - |
| H7994_RS24370 | 94061..94135 | - | 75 | WP_001442103.1 | RepA leader peptide Tap | - |
| H7994_RS24375 | 94367..94624 | - | 258 | WP_000083845.1 | replication regulatory protein RepA | - |
| H7994_RS24380 | 94908..95114 | - | 207 | WP_001351576.1 | type I toxin-antitoxin system Hok family toxin | - |
| - | 95101..95162 | + | 62 | NuclAT_1 | - | - |
| - | 95101..95162 | + | 62 | NuclAT_1 | - | - |
| - | 95101..95162 | + | 62 | NuclAT_1 | - | - |
| - | 95101..95162 | + | 62 | NuclAT_1 | - | - |
| H7994_RS24385 | 95405..96241 | - | 837 | WP_000480968.1 | aminoglycoside O-phosphotransferase APH(6)-Id | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | aph(6)-Id / aph(3'')-Ib / blaTEM-1B / tet(A) / aph(3')-IIa / sitABCD | iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA / iroB / iroC | 1..237102 | 237102 | |
| - | inside | IScluster/Tn | aph(6)-Id / aph(3'')-Ib / blaTEM-1B / tet(A) / aph(3')-IIa | - | 87084..122647 | 35563 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11105.15 Da Isoelectric Point: 10.5832
>T182166 WP_000222760.1 NZ_CP065153:c91526-91239 [Escherichia coli O150:H6]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
Download Length: 288 bp
>T182166 NZ_CP086336:c3941172-3940858 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9211.41 Da Isoelectric Point: 4.0780
>AT182166 WP_001132900.1 NZ_CP065153:c91774-91523 [Escherichia coli O150:H6]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
Download Length: 252 bp
>AT182166 NZ_CP086336:c3940861-3940445 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|