Detailed information of TA system TA182165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 31031..31407
• Replicon: plasmid unnamed1
• Accession: NZ_CP065153
• Organism: Escherichia coli O150:H6 strain FEX669

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: H7994_RS23985
• Protein ID: WP_000969988.1
• Coordinates: 31031..31213 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: H7994_RS23990
• Protein ID: WP_001324224.1
• Coordinates: 31210..31407 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7994_RS23975	29030..30064	-	1035	WP_001190233.1	3-deoxy-7-phosphoheptulonate synthase	-
H7994_RS23980	30624..30932	-	309	WP_000377483.1	hypothetical protein	-
H7994_RS23985	31031..31213	-	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7994_RS23990	31210..31407	-	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
H7994_RS23995	32089..33363	+	1275	WP_000489608.1	HlyD family secretion protein	-
H7994_RS24000	33338..35452	+	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
H7994_RS24005	35622..35705	-	84	Protein_26	colicin V	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / blaTEM-1B / tet(A) / aph(3')-IIa / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA / iroB / iroC	1..237102	237102

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Antitoxin MqsA/HigA-2 (1-131)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T182165 WP_000969988.1 NZ_CP065153:c31213-31031 [Escherichia coli O150:H6]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T182165 NZ_CP086336:c3876597-3876301 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT182165 WP_001324224.1 NZ_CP065153:c31407-31210 [Escherichia coli O150:H6]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT182165 NZ_CP086336:c3876299-3875904 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A0A075MDK3