Detailed information of TA system TA182155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3822626..3824214
• Replicon: chromosome
• Accession: NZ_CP065152
• Organism: Escherichia coli O150:H6 strain FEX669

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: H7994_RS18420
• Protein ID: WP_001125428.1
• Coordinates: 3822626..3823948 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V0VAH2
• Locus tag: H7994_RS18425
• Protein ID: WP_001304368.1
• Coordinates: 3823948..3824214 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7994_RS18385	3818393..3818626	-	234	WP_000473544.1	hypothetical protein	-
H7994_RS18390	3818661..3818912	-	252	WP_000381239.1	membrane protein	-
H7994_RS18395	3818915..3819232	-	318	WP_000073835.1	hypothetical protein	-
H7994_RS18400	3819244..3820296	-	1053	WP_000568109.1	hypothetical protein	-
H7994_RS18405	3820468..3820914	+	447	WP_000527682.1	helix-turn-helix domain-containing protein	-
H7994_RS18410	3821014..3821433	+	420	WP_000089315.1	hypothetical protein	-
H7994_RS18415	3821520..3822449	+	930	WP_000963948.1	hypothetical protein	-
H7994_RS18420	3822626..3823948	-	1323	WP_001125428.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
H7994_RS18425	3823948..3824214	-	267	WP_001304368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
H7994_RS18430	3824522..3825280	+	759	WP_001286540.1	trans-aconitate 2-methyltransferase	-
H7994_RS18435	3825284..3826198	-	915	WP_121831908.1	bestrophin family protein	-
H7994_RS18440	3826395..3827846	-	1452	WP_000854637.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	3771235..3824214	52979

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49331.98 Da
• Isoelectric Point: 8.1297

>T182155 WP_001125428.1 NZ_CP065152:c3823948-3822626 [Escherichia coli O150:H6]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
FRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T182155 NZ_CP086336:c1916699-1916592 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9957.31 Da
• Isoelectric Point: 4.9632

>AT182155 WP_001304368.1 NZ_CP065152:c3824214-3823948 [Escherichia coli O150:H6]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Nucleotide

• Length: 267 bp

>AT182155 NZ_CP086336:1916746-1916812 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: V0VAH2