Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3326245..3327043 Replicon chromosome
Accession NZ_CP065152
Organism Escherichia coli O150:H6 strain FEX669

Toxin (Protein)


Gene name yeeV Uniprot ID A7ZRI1
Locus tag H7994_RS15825 Protein ID WP_000854738.1
Coordinates 3326666..3327043 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A444R4B6
Locus tag H7994_RS15820 Protein ID WP_021531223.1
Coordinates 3326245..3326619 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7994_RS15780 3322257..3322712 + 456 Protein_3066 hypothetical protein -
H7994_RS15785 3322831..3323042 - 212 Protein_3067 hypothetical protein -
H7994_RS15790 3323123..3323944 + 822 WP_196315955.1 DUF945 domain-containing protein -
H7994_RS15795 3323944..3324189 + 246 WP_001164966.1 hypothetical protein -
H7994_RS15800 3324283..3324756 + 474 WP_196315956.1 antirestriction protein -
H7994_RS15805 3324772..3325248 + 477 WP_001186774.1 RadC family protein -
H7994_RS15810 3325311..3325532 + 222 WP_000692307.1 DUF987 domain-containing protein -
H7994_RS15815 3325551..3326195 + 645 WP_196315957.1 antitoxin of toxin-antitoxin stability system -
H7994_RS15820 3326245..3326619 + 375 WP_021531223.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
H7994_RS15825 3326666..3327043 + 378 WP_000854738.1 TA system toxin CbtA family protein Toxin
H7994_RS15830 3327040..3327543 + 504 WP_021531035.1 hypothetical protein -
H7994_RS15835 3327540..3327737 + 198 WP_000839287.1 DUF957 domain-containing protein -
H7994_RS15840 3327834..3328661 + 828 WP_021531224.1 DUF4942 domain-containing protein -
H7994_RS15850 3329148..3330086 + 939 WP_097520840.1 glyoxylate/hydroxypyruvate reductase GhrA -
H7994_RS15855 3330141..3330878 + 738 WP_196315958.1 phosphatase -
H7994_RS15860 3330902..3331456 + 555 WP_001001921.1 molecular chaperone YcdY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 3301600..3337612 36012


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14089.12 Da        Isoelectric Point: 8.2904

>T182149 WP_000854738.1 NZ_CP065152:3326666-3327043 [Escherichia coli O150:H6]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMVRDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T182149 NZ_CP086336:392050-392400 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13763.43 Da        Isoelectric Point: 4.7511

>AT182149 WP_021531223.1 NZ_CP065152:3326245-3326619 [Escherichia coli O150:H6]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT182149 NZ_CP086336:391805-392056 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A7ZRI1

Antitoxin

Source ID Structure
AlphaFold DB A0A444R4B6

References