Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2264196..2264746 Replicon chromosome
Accession NZ_CP065152
Organism Escherichia coli O150:H6 strain FEX669

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag H7994_RS10825 Protein ID WP_001160966.1
Coordinates 2264432..2264746 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag H7994_RS10820 Protein ID WP_000125566.1
Coordinates 2264196..2264429 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7994_RS10795 2259269..2259556 + 288 WP_000203741.1 ferredoxin-like protein FixX -
H7994_RS10800 2259615..2260946 + 1332 WP_001183207.1 MFS transporter -
H7994_RS10805 2261054..2261584 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
H7994_RS10810 2261577..2263439 + 1863 WP_001591511.1 glutathione-regulated potassium-efflux system protein KefC -
H7994_RS10815 2263631..2264110 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
H7994_RS10820 2264196..2264429 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H7994_RS10825 2264432..2264746 + 315 WP_001160966.1 CcdB family protein Toxin
H7994_RS10830 2264743..2265591 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
H7994_RS10835 2265598..2265975 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
H7994_RS10840 2265978..2266799 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
H7994_RS10845 2266796..2267785 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
H7994_RS10850 2267785..2269071 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T182146 WP_001160966.1 NZ_CP065152:2264432-2264746 [Escherichia coli O150:H6]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T182146 NZ_CP086334:4491368-4491703 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT182146 WP_000125566.1 NZ_CP065152:2264196-2264429 [Escherichia coli O150:H6]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT182146 NZ_CP086334:4491120-4491368 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References