Detailed information of TA system TA182141

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 499366..500201
• Replicon: chromosome
• Accession: NZ_CP065152
• Organism: Escherichia coli O150:H6 strain FEX669

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: H7994_RS02480
• Protein ID: WP_087890264.1
• Coordinates: 499824..500201 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A085P7I7
• Locus tag: H7994_RS02475
• Protein ID: WP_001327592.1
• Coordinates: 499366..499734 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7994_RS02450	496043..496498	+	456	WP_000581502.1	hypothetical protein	-
H7994_RS02455	496782..497603	+	822	WP_160520991.1	DUF945 domain-containing protein	-
H7994_RS02460	497942..498421	+	480	WP_019842849.1	antirestriction protein	-
H7994_RS02465	498437..498913	+	477	WP_001186727.1	RadC family protein	-
H7994_RS02470	498982..499203	+	222	WP_000692300.1	DUF987 domain-containing protein	-
H7994_RS02475	499366..499734	+	369	WP_001327592.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H7994_RS02480	499824..500201	+	378	WP_087890264.1	TA system toxin CbtA family protein	Toxin
H7994_RS02485	500198..500686	+	489	WP_044186373.1	hypothetical protein	-
H7994_RS02490	500698..500895	+	198	WP_044186375.1	DUF957 domain-containing protein	-
H7994_RS02495	500980..501645	+	666	WP_196316103.1	DUF4942 domain-containing protein	-
H7994_RS02500	501591..501821	+	231	Protein_488	DUF4942 domain-containing protein	-
H7994_RS02505	502163..502333	+	171	Protein_489	IS110 family transposase	-
H7994_RS02510	503144..504127	+	984	WP_001331698.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	502229..502333	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14329.23 Da
• Isoelectric Point: 9.6859

>T182141 WP_087890264.1 NZ_CP065152:499824-500201 [Escherichia coli O150:H6]
MNTLPDTHVREASRSPSPVTIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRF
STSPRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKR

Nucleotide

• Length: 378 bp

>T182141 NZ_CP086334:c3992691-3992227 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13901.65 Da
• Isoelectric Point: 6.3165

>AT182141 WP_001327592.1 NZ_CP065152:499366-499734 [Escherichia coli O150:H6]
VSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT182141 NZ_CP086334:c3993026-3992691 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A085P7I7