Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 341687..342341 Replicon chromosome
Accession NZ_CP065152
Organism Escherichia coli O150:H6 strain FEX669

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag H7994_RS01710 Protein ID WP_000244781.1
Coordinates 341687..342094 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H7994_RS01715 Protein ID WP_000354046.1
Coordinates 342075..342341 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7994_RS01690 337644..339377 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
H7994_RS01695 339383..340093 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H7994_RS01700 340118..341014 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H7994_RS01705 341126..341647 + 522 WP_001055874.1 flavodoxin FldB -
H7994_RS01710 341687..342094 - 408 WP_000244781.1 protein YgfX Toxin
H7994_RS01715 342075..342341 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H7994_RS01720 342584..343564 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
H7994_RS01725 343641..344300 - 660 WP_000250274.1 hemolysin III family protein -
H7994_RS01730 344464..344775 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
H7994_RS01735 344820..346253 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T182140 WP_000244781.1 NZ_CP065152:c342094-341687 [Escherichia coli O150:H6]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T182140 NZ_CP086334:3584797-3584904 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT182140 WP_000354046.1 NZ_CP065152:c342341-342075 [Escherichia coli O150:H6]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT182140 NZ_CP086334:c3584748-3584685 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References