Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 187415..187998 Replicon chromosome
Accession NZ_CP065152
Organism Escherichia coli O150:H6 strain FEX669

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag H7994_RS01080 Protein ID WP_000254750.1
Coordinates 187415..187750 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag H7994_RS01085 Protein ID WP_000581937.1
Coordinates 187750..187998 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7994_RS01065 183301..184599 - 1299 WP_000036723.1 phosphopyruvate hydratase -
H7994_RS01070 184687..186324 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
H7994_RS01075 186552..187343 - 792 WP_001071677.1 nucleoside triphosphate pyrophosphohydrolase -
H7994_RS01080 187415..187750 - 336 WP_000254750.1 endoribonuclease MazF Toxin
H7994_RS01085 187750..187998 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
H7994_RS01090 188076..190310 - 2235 WP_000226815.1 GTP pyrophosphokinase -
H7994_RS01095 190358..191659 - 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T182139 WP_000254750.1 NZ_CP065152:c187750-187415 [Escherichia coli O150:H6]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T182139 NZ_CP086334:3584314-3584421 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT182139 WP_000581937.1 NZ_CP065152:c187998-187750 [Escherichia coli O150:H6]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT182139 NZ_CP086334:c3584265-3584202 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References