Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2320594..2321569 Replicon chromosome
Accession NZ_CP065149
Organism Bacillus paranthracis strain Gxun-30

Toxin (Protein)


Gene name spoIISA Uniprot ID C2MKV2
Locus tag I0K14_RS11840 Protein ID WP_001997496.1
Coordinates 2320594..2321331 (+) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A402GGJ7
Locus tag I0K14_RS11845 Protein ID WP_000594783.1
Coordinates 2321444..2321569 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I0K14_RS11810 2315679..2316389 + 711 WP_000787480.1 class I SAM-dependent methyltransferase -
I0K14_RS11815 2316509..2316916 + 408 WP_000072485.1 VOC family protein -
I0K14_RS11820 2317022..2317114 + 93 Protein_2245 methyltransferase -
I0K14_RS11825 2317236..2318921 - 1686 WP_033670814.1 alpha-keto acid decarboxylase family protein -
I0K14_RS11830 2319018..2319500 + 483 WP_000191884.1 MarR family winged helix-turn-helix transcriptional regulator -
I0K14_RS11835 2319668..2320405 + 738 WP_000594124.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
I0K14_RS11840 2320594..2321331 + 738 WP_001997496.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
I0K14_RS11845 2321444..2321569 + 126 WP_000594783.1 hypothetical protein Antitoxin
I0K14_RS11850 2321645..2321821 + 177 WP_000819051.1 hypothetical protein -
I0K14_RS11855 2321865..2322140 - 276 WP_001041552.1 hypothetical protein -
I0K14_RS11860 2322699..2322875 + 177 WP_000852621.1 hypothetical protein -
I0K14_RS11865 2322899..2323288 - 390 WP_000808872.1 YxeA family protein -
I0K14_RS11870 2323504..2324973 + 1470 WP_000287532.1 beta-Ala-His dipeptidase -
I0K14_RS11875 2325554..2326024 - 471 WP_000771836.1 DUF5065 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(9-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28377.08 Da        Isoelectric Point: 8.2672

>T182134 WP_001997496.1 NZ_CP065149:2320594-2321331 [Bacillus paranthracis]
VISNIRIGLFILAIVFLVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTMIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T182134 NZ_CP086334:c2769829-2769539 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4879.29 Da        Isoelectric Point: 3.9798

>AT182134 WP_000594783.1 NZ_CP065149:2321444-2321569 [Bacillus paranthracis]
VIKNSDNDFFKEDNEDTTDFSLIKGDTMQQVEELEEELKRK

Download         Length: 126 bp

>AT182134 NZ_CP086334:c2770061-2769819 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1T2Q3U8

Antitoxin

Source ID Structure
AlphaFold DB A0A402GGJ7

References