Detailed information of TA system TA182130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2020658..2021457
• Replicon: chromosome
• Accession: NZ_CP065148
• Organism: Proteus mirabilis strain PmBJ012

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E3RD10
• Locus tag: I0U42_RS09345
• Protein ID: WP_004247966.1
• Coordinates: 2020658..2021182 (-)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B4EVS2
• Locus tag: I0U42_RS09350
• Protein ID: WP_004247964.1
• Coordinates: 2021179..2021457 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0U42_RS09330	2016974..2017915	-	942	WP_004242695.1	glyoxylate/hydroxypyruvate reductase GhrA	-
I0U42_RS09340	2018877..2020577	+	1701	WP_004247967.1	C4-dicarboxylic acid transporter DauA	-
I0U42_RS09345	2020658..2021182	-	525	WP_004247966.1	hypothetical protein	Toxin
I0U42_RS09350	2021179..2021457	-	279	WP_004247964.1	DUF1778 domain-containing protein	Antitoxin
I0U42_RS09355	2021535..2022464	-	930	WP_004242691.1	TIGR01212 family radical SAM protein	-
I0U42_RS09360	2022472..2023326	-	855	WP_004242689.1	3-deoxy-8-phosphooctulonate synthase	-
I0U42_RS09365	2023384..2024199	-	816	WP_004242688.1	invasion regulator SirB1	-
I0U42_RS09370	2024177..2025025	-	849	WP_004247962.1	peptide chain release factor N(5)-glutamine methyltransferase	-
I0U42_RS09375	2025025..2026107	-	1083	WP_004242680.1	peptide chain release factor 1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19192.12 Da
• Isoelectric Point: 9.3561

>T182130 WP_004247966.1 NZ_CP065148:c2021182-2020658 [Proteus mirabilis]
MTIPNWHEEAISKKHNRNGFNCGDAVLNQFLYRHARQNHENGSAKTYLAINNSNNIILGYYSLCPASIEFERTPELIKHG
LARHDIPVFKLARLATDLSVQGKGLGGQLLLAAGRRCLSVASELGGVALLIDAKNERVANWYISYGAIPLLDTPLSLLIS
FKTIYTALSMANKI

Nucleotide

• Length: 525 bp

>T182130 NZ_CP086334:2024950-2025168 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10252.82 Da
• Isoelectric Point: 5.1369

>AT182130 WP_004247964.1 NZ_CP065148:c2021457-2021179 [Proteus mirabilis]
MAQALVENSERVSIRITTEDKSLLVRAASLENTNLTEFVLRNIVPVARQVIENNERLKLSEKDTLRVMELLDNPPKPNAK
LLAAAFALPDNA

Nucleotide

• Length: 279 bp

>AT182130 NZ_CP086334:2024550-2024924 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: B4EVS2