Detailed information of TA system TA182113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2214631..2215652
• Replicon: chromosome
• Accession: NZ_CP065145
• Organism: Proteus mirabilis strain PmBJ023

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: I0U43_RS10005
• Protein ID: WP_004249488.1
• Coordinates: 2214631..2215200 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: I0U43_RS10010
• Protein ID: WP_004245528.1
• Coordinates: 2215200..2215652 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0U43_RS10000	2212161..2214515	-	2355	WP_049199421.1	DNA polymerase II	-
I0U43_RS10005	2214631..2215200	-	570	WP_004249488.1	PIN domain-containing protein	Toxin
I0U43_RS10010	2215200..2215652	-	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
I0U43_RS10015	2215780..2216481	-	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
I0U43_RS10020	2216474..2218054	-	1581	WP_004245526.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
I0U43_RS10025	2218054..2219067	-	1014	WP_121910087.1	thiamine ABC transporter substrate binding subunit	-
I0U43_RS10030	2219336..2219866	+	531	WP_004245524.1	RNA pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21600.10 Da
• Isoelectric Point: 7.3470

>T182113 WP_004249488.1 NZ_CP065145:c2215200-2214631 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKNPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T182113 NZ_CP086333:c1086557-1086300 [Xanthomonas translucens pv. arrhenatheri]
GTGACGGTTCAGTTCTCGGACAATGCCTGGGAGGACTACCTCTATTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATTCAATGCGATCCCTTCCAGGGCGTCGGCAAGCCAGAACCGCTGCGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACACCGCATCGTGTACAAGGTCGAGGCGGGCGTCCTGCTGATCGCCCAAGTG
CGGTATCACTACGCGTAG

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT182113 WP_004245528.1 NZ_CP065145:c2215652-2215200 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT182113 NZ_CP086333:c1086757-1086554 [Xanthomonas translucens pv. arrhenatheri]
ATGGAGCGTGTCGTCAACAATCACGAGCCGGTGATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTTGA
GGACTACAAGGCGATGGAGGAAACCGCCTACCTGCTCCGCAGTCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCC
AGCTCGAGGCCGGTCGCGGCAAGGCACGGGACCTGGCCGAGTGA

Structures

Antitoxin

• AlphaFold DB: B4F2H3