Detailed information of TA system TA182109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-MqsA
• Location: 1298070..1298769
• Replicon: chromosome
• Accession: NZ_CP065145
• Organism: Proteus mirabilis strain PmBJ023

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B4EZ96
• Locus tag: I0U43_RS05680
• Protein ID: WP_004249093.1
• Coordinates: 1298070..1298456 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B4EZ97
• Locus tag: I0U43_RS05685
• Protein ID: WP_004246828.1
• Coordinates: 1298449..1298769 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0U43_RS05665	1294089..1294670	-	582	WP_049198882.1	DNA-3-methyladenine glycosylase I	-
I0U43_RS05670	1294921..1295826	+	906	WP_004246832.1	glycine--tRNA ligase subunit alpha	-
I0U43_RS05675	1295836..1297908	+	2073	WP_004246830.1	glycine--tRNA ligase subunit beta	-
I0U43_RS05680	1298070..1298456	+	387	WP_004249093.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I0U43_RS05685	1298449..1298769	+	321	WP_004246828.1	helix-turn-helix domain-containing protein	Antitoxin
I0U43_RS05690	1298818..1299252	-	435	WP_004246827.1	hypothetical protein	-
I0U43_RS05695	1299245..1300129	-	885	WP_012368526.1	endonuclease/exonuclease/phosphatase family protein	-
I0U43_RS05700	1300346..1301632	-	1287	WP_017628657.1	DUF3748 domain-containing protein	-
I0U43_RS05705	1301769..1302386	+	618	WP_004246822.1	trimeric intracellular cation channel family protein	-
I0U43_RS05710	1302688..1303311	+	624	WP_004246821.1	guanylate kinase	-
I0U43_RS05715	1303366..1303641	+	276	WP_004246820.1	DNA-directed RNA polymerase subunit omega	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-95)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14294.64 Da
• Isoelectric Point: 10.0642

>T182109 WP_004249093.1 NZ_CP065145:1298070-1298456 [Proteus mirabilis]
MVIYKTKMFKNSFKKITLTNAELIAIATEVLAGQFEADLGGGVIKKRACIQGKGKSSGIRTIIFYKQGNNLFFADGWKKS
SLSSKKTKEITDDELESYKDLAKDLFNANQNKIDKMIALGLLTEVKYD

Nucleotide

• Length: 387 bp

>T182109 NZ_CP086332:3687984-3688241 [Xanthomonas translucens pv. phleipratensis]
GTGACGGTTCAGTTCTCGGACAATGCCTGGGAGGACGACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAACGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAAGGCGTCGGCAAGCCAGAACCGCTACGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACACCGCATCGTGTACAAGGTCGAGGCGGGCGTCCTGCTGATCGCGCAGGTG
CGCTACCACTACGTGTAG

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12126.21 Da
• Isoelectric Point: 10.4411

>AT182109 WP_004246828.1 NZ_CP065145:1298449-1298769 [Proteus mirabilis]
MTDKMKEMLKFAQEMEDAGVTDGSTTKRIAARIKAKELQAKYKHPTPMTGEDIKRIREQNNISQSSLALILNMTKESVSK
WERNEIKPNGAALRLLHLIDKKILFI

Nucleotide

• Length: 321 bp

>AT182109 NZ_CP086332:3687736-3687987 [Xanthomonas translucens pv. phleipratensis]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTCGCGGACACCATGGAGCGTGTCGTCAACAATCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTTGAGGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTCCGCAGTCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTCGAGGCCGGTCGCGGCAAGGCACGGGAT
CTGGCCGAGTGA

Structures

Toxin

• AlphaFold DB: B4EZ96

Antitoxin

• AlphaFold DB: B4EZ97