Detailed information of TA system TA182102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3179207..3180124
• Replicon: chromosome
• Accession: NZ_CP065137
• Organism: Bacillus sp. A1(2020)

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: IRJ20_RS16210
• Protein ID: WP_007407256.1
• Coordinates: 3179378..3180124 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: IRJ20_RS16205
• Protein ID: WP_003154807.1
• Coordinates: 3179207..3179377 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRJ20_RS16165	3174430..3176061	+	1632	WP_015417294.1	pyocin knob domain-containing protein	-
IRJ20_RS16170	3176074..3176445	+	372	WP_015417295.1	XkdW family protein	-
IRJ20_RS16175	3176450..3176647	+	198	WP_012117366.1	XkdX family protein	-
IRJ20_RS16180	3176704..3177465	+	762	WP_033575307.1	hypothetical protein	-
IRJ20_RS16185	3177517..3177780	+	264	WP_003154815.1	hemolysin XhlA family protein	-
IRJ20_RS16190	3177794..3178057	+	264	WP_003154813.1	phage holin	-
IRJ20_RS16195	3178071..3178949	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
IRJ20_RS16205	3179207..3179377	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
IRJ20_RS16210	3179378..3180124	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
IRJ20_RS16215	3180228..3181226	-	999	WP_003154805.1	inorganic phosphate transporter	-
IRJ20_RS16220	3181239..3181856	-	618	WP_003154804.1	DUF47 domain-containing protein	-
IRJ20_RS16225	3182142..3183458	-	1317	WP_007610842.1	amino acid permease	-
IRJ20_RS16230	3183781..3184731	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T182102 WP_007407256.1 NZ_CP065137:c3180124-3179378 [Bacillus sp. A1(2020)]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T182102 NZ_CP086326:387092-387475 [Lacticaseibacillus rhamnosus]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAACAATGTTGGTAACCATTATAGCCCCACTGTTATCGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACCCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGTTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
GGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT182102 WP_003154807.1 NZ_CP065137:c3179377-3179207 [Bacillus sp. A1(2020)]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT182102 NZ_CP086326:386824-387072 [Lacticaseibacillus rhamnosus]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGCGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAATTGATTT
CTGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14